Leukemia inhibitor factor in skeletal muscle regrowth

骨骼肌再生中的白血病抑制因子

• 批准号: 6445216
• 负责人: ESPEN E SPANGENBURG
• 金额: $ 3.78万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-08 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6445216
• 关键词: atrophy; biological signal transduction; cell proliferation; immobilization of body part; immunoprecipitation; laboratory rat; leg; leukemia inhibitory factor; muscle satellite cell; phosphatidylinositol 3 kinase; regeneration; striated muscles; tissue /cell culture

Increasing skeletal muscle growth after an induction of an atrophying inducing stimulus (i.e. disuse) is extremely important in various medical conditions. Skeletal muscle wasting can contribute to increased health care costs and a decreased quality of life. The proposed research would examine the mechanism that allows Leukemia Inhibitory Factor (LIF) to inducer satellite cell proliferation and the role it may play in muscle re- growth after 10 days of immobilization. This research would be novel in that it would identify a possible signaling mechanism that may regulate satellite cell proliferation and ultimately affect skeletal muscle growth. Further, the research would extend the role of a cytokine known to cause cardiac hypertrophy to skeletal muscle. This would be a completely novel finding. Pilot data that I have colleted indicates that LIF can induce myoblast proliferation, possibly by activating the NAK2-STAT2 or PI3K pathways. Also, I found that skeletal muscle expressed the LIF protein after 6 days of ambulatory recovery from 10 days of hindlimb immobilization. Therefore, it is proposed to determine which signaling cascades are necessary for LIF-indued satellite proliferation and to determine if this pathway extends to the whole animal. Identification of a cytokine and a signaling mechanism that may regulate muscle growth could lead to other counter-measures to ultimately prevent muscle wasting.

在诱导萎缩诱导刺激（即废用）之后增加骨骼肌生长在各种医学状况中是极其重要的。骨骼肌萎缩会导致医疗费用增加和生活质量下降。拟议的研究将检查允许白血病抑制因子（LIF）诱导卫星细胞增殖的机制，以及它在固定10天后可能在肌肉重新生长中发挥的作用。这项研究将是新颖的，因为它将确定一个可能的信号机制，可以调节卫星细胞增殖，并最终影响骨骼肌的生长。此外，这项研究将扩大已知导致心肌肥大的细胞因子对骨骼肌的作用。这将是一个全新的发现。我收集的初步数据表明，LIF可以诱导成肌细胞增殖，可能是通过激活NAK 2-STAT 2或PI 3 K途径。此外，我发现骨骼肌表达LIF蛋白后6天的步行恢复10天的后肢制动。 因此，建议确定哪些信号级联是LIF诱导的卫星增殖所必需的，并确定该途径是否延伸到整个动物。确定一种细胞因子和一种可能调节肌肉生长的信号传导机制，可能会导致其他对策，最终防止肌肉萎缩。