Effect of amiloride and gurmarin on taste receptor cells

描述（由申请人提供）：拟定研究旨在 研究特定味觉输入到神经系统的排列 在接收器阵列中。当前的目标是了解 阿米洛利和香豆素敏感性转导机制在 味觉感受器细胞（taste receptor cells，TRC）分布于菌状上皮和腭部上皮。 为了实现这些目标，选择了一种实验范式， 允许从基底外侧膜记录跨膜电流 在味觉刺激后，仅限于 粘膜表面。因此，该过程允许化学刺激TRCs 以一种代表这些细胞如何自然相互作用的方式 与味觉刺激，这提供了一个公正的方法来衡量调谐 储税券的概况。此外，该制剂理想地适合于测定 阿米洛利和香豆素对味觉敏感性的影响 个人储税券。再加上已知的这些影响， 高级神经元元素上的物质，从建议的 实验可能会从逻辑上揭示特定突触链接的存在 在特定类型的TRCs和表达共同的 在特定味觉输入阻断后，敏感度发生变化。

DESCRIPTION (provided by applicant): The proposed research is designed to investigate the arrangement of specific gustatory inputs to the nervous system within the receptor array. The immediate objective is to understand the distribution of amiloride- and gurmarin-sensitive transduction mechanisms across taste receptor cells (TRCs) located in both the fungiform and palatal epithelia. Toward achievement of these aims, an experimental paradigm was chosen that allows transmembrane currents to be patch recorded from the basolateral membrane of individual TRCs in situ following taste stimulation that is restricted to the mucosal surface. Thus, this procedure permits one to chemically stimulate TRCs in a manner that is representative of how these cells would naturally interact with taste stimuli, which provides an unbiased method for measuring the tuning profiles of TRCs. Moreover, this preparation is ideally suited for determining the influence of both amiloride and gurmarin on the gustatory sensitivities of individual TRCs. When coupled with what is known regarding the effects of these substances on higher-order neuronal elements, data obtained from the proposed experiments may logically reveal the existence of specific synaptic links between particular types of TRCs and nerve fibers that express common sensitivity shifts following specific gustatory input blockage.