FUNCTION OF THE BACULOVIRUS MEMBRANE FUSION PROTEIN

杆状病毒膜融合蛋白的功能

• 批准号: 6510643
• 负责人: GARY W BLISSARD
• 金额: $ 16.85万
• 依托单位: BOYCE THOMPSON INST FOR PLANT RESEARCH
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6510643
• 关键词: Baculoviridae; X ray crystallography; binding proteins; fluorescence microscopy; gene mutation; immunoelectron microscopy; immunoprecipitation; membrane fusion; membrane proteins; molecular cloning; nucleic acid sequence; protein structure function; receptor binding; site directed mutagenesis; tissue /cell culture; virus assembly; virus envelope; virus infection mechanism; virus protein; virus replication; yeast two hybrid system

DESCRIPTION (Adapted from the Applicant's Abstract): Baculoviruses are highly virulent insect viruses that serve as biological control agents and as important expression vectors. As pathogenic insect viruses, baculoviruses serve as excellent model systems for studies of viral interactions with insect tissues and cells. Our studies have focused on the major envelope protein, GP64, of the budded virus particle. The GP64 protein appears to serve a number of functions in the infection cycle: 1) recognition of the host cell receptor molecules; 2) fusion of viral and host membranes during virus entry; and, 3) participation in budding or assembly of the virus.

描述（改编自申请人的摘要）：杆状病毒是高毒性昆虫病毒，其用作生物防治剂和重要的表达载体。杆状病毒作为昆虫病原病毒，是研究病毒与昆虫组织细胞相互作用的理想模型系统。我们的研究主要集中在出芽病毒颗粒的主要包膜蛋白GP 64上。GP 64蛋白似乎在感染周期中发挥许多功能：1）识别宿主细胞受体分子; 2）在病毒进入期间融合病毒和宿主膜;以及3）参与病毒的出芽或组装。