Mechanisms of uptake anof uptake and storage of [trace metals] by Miscanthus - implications for growing a commercial fuel crop on contaminated land.

芒草吸收和储存[微量金属]的机制——对在受污染土地上种植商业燃料作物的影响。

• 批准号: 1948270
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1948270
• 关键词: Mechanisms; uptake; anof; storage; trace

This project would examine the fundamental mechanisms of the uptake of trace metals by Miscanthus grown on contaminated land as a commercial biomass crop. It will seek to establish a quantitative and qualitative 'Substance Flow Analysis' of trace metals from soils into the plants, their distribution/storage within plant structures, and the chemical/bio-chemical species underpinning this process. Objectives:The outline objectives of the research would be: Identify specific trace metal(s) for study of relevance to an identified contaminated site;Select hybrids suitable for study (resistance, active uptake, commercially relevant); Establish an 'in vitro' growth trial of plants in the laboratory using an engineered growing medium;Conduct detailed analysis of mechanisms/chemistry responsible for trace metals uptake and storage within the plants; andUnderstand the implications for use of the biomass as a fuel, its technical suitability for different end users and where pre-treatment may be necessary.Project Delivery: The research would be delivered with industry support from Terravesta Ltd - a commercial supplier of Miscanthus plants, biomass processor and biomass fuel supplier with interests in the UK and Poland. Terravesta would provide seeds/rhizomes (tbc) of hybrid species considered of interest. The trace metals in question will be selected based on characterisation of Terravesta's case study sites in Poland and the contamination therein. The shortlist of species is likely to include Cd, Zn, Pb and As. The project will have two principal work streams working in parallel as follows:1) Laboratory grown plants: would be grown in a laboratory (Civ. Eng.) in a controlled 'engineered' growing substrate designed to allow observation of the selected trace metals. These plants would be subject to detailed study and analysis (below). 2) Field study plants: would be periodically sampled from Miscanthus grown on Terravesta's sites in Poland. A simplified analytical suite will be developed to 'cross-check and calibrate' the laboratory results with a 'real world' application. The above 'twin track' approach will ensure the project delivers practical results with demonstrable impacts beyond the laboratory. Analytical Techniques:The analyses to be adopted will depend on the finalised list of trace metals and the finalised analytical plan (to be developed). In principle, some examples of techniques that may be applicable include:General fuel characterisation (e.g. TGA, IC/major inorganics);Quantitative elemental analysis of trace metals (e.g. AAS, ICP-MS, XRF);Quantitative analysis of metal species (e.g. synchrotron XAS) Pre-RequisitesFollowing initial meetings with supervisors and Terravesta the following pre-requisites have been identified for this research:Non-Disclosure Agreement (nb. Terravesta have signed the university's standard terms and this needs to be counter signed and returned to them)Clarification/confirmation of contribution 'in kind' expected from Terravesta Material Transfer Agreement (to be developed)Data Management Plan (to be developed)Site visit to Poland (to be arranged for autumn '18).

该项目将研究作为商业生物质作物种植在受污染土地上的芒草吸收微量金属的基本机制。它将寻求对从土壤到植物的痕量金属、它们在植物结构内的分布/储存以及支撑这一过程的化学/生物化学物种建立定量和定性的“物质流分析”。目标：研究的总体目标是： 识别特定的痕量金属，以研究与已识别的污染场地的相关性；选择适合研究的杂种（抗性、主动吸收、商业相关）；使用工程生长培养基在实验室中建立植物的“体外”生长试验；对植物内微量金属吸收和储存的机制/化学进行详细分析；并了解使用生物质作为燃料的影响、其对不同最终用户的技术适用性以及可能需要预处理的情况。 项目交付：该研究将在 Terravesta Ltd 的行业支持下交付，Terravesta Ltd 是芒草植物、生物质加工商和生物质燃料供应商的商业供应商，在英国和波兰有兴趣。 Terravesta 将提供感兴趣的杂交物种的种子/根茎（tbc）。将根据 Terravesta 在波兰的案例研究地点的特征及其污染情况来选择所讨论的痕量金属。入围名单可能包括镉、锌、铅和砷。该项目将有两个并行工作的主要工作流程，如下：1）实验室种植的植物：将在实验室（民用工程）中在受控的“工程”生长基质中生长，该基质旨在允许观察选定的痕量金属。这些植物将接受详细的研究和分析（如下）。 2) 实地研究植物：定期从波兰 Terravesta 工厂种植的芒草中取样。将开发一个简化的分析套件，以通过“现实世界”应用程序“交叉检查和校准”实验室结果。上述“双轨”方法将确保该项目取得实际成果，并在实验室之外产生明显的影响。分析技术：所采用的分析将取决于最终确定的痕量金属清单和最终确定的分析计划（待制定）。原则上，可能适用的一些技术示例包括：一般燃料表征（例如 TGA、IC/主要无机物）；痕量金属的定量元素分析（例如 AAS、ICP-MS、XRF）；金属种类的定量分析（例如同步加速器 XAS）先决条件在与主管和 Terravesta 进行初步会议后，已确定了以下先决条件： 研究：保密协议（注：Terravesta 已签署大学的标准条款，需要会签并返回给他们）对 Terravesta 材料转让协议（待制定）“实物”贡献的澄清/确认（待制定）数据管理计划（待制定）对波兰进行实地考察（将于 18 年秋季安排）。