SPATIALLY SELECTIVE COAGULATION OF HYPERVASCULAR LESIONS

高血管病变的空间选择性凝固

• 批准号: 6512133
• 负责人: BAHMAN ANVARI
• 金额: $ 23.27万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-19 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6512133
• 关键词: blood vessel disorder; body temperature; cardiovascular disorder therapy; chickens; clinical trials; cold temperature; combination therapy; cryoprotective agents; cryoscience; disease /disorder model; drug screening /evaluation; histology; human subject; human therapy evaluation; lasers; mathematical model; model design /development; nonhuman therapy evaluation; patient oriented research; photocoagulation therapy; radiation dosage; skin disorder; skin disorder chemotherapy; thermodynamics; whole body irradiation dosage

DESCRIPTION (Verbatim from Applicant's Abstract): Laser treatment of hypervascular cutaneous malformations such as port wine stain (PWS) lesion is based or selective photocoagulation of subsurface targeted blood vessels without inducing thermal damage to the superficial skin layers. The principal investigator and his collaborators are interested in development and optimization of cryogen spray cooling (CSC) to protect the superficial skin layers from thermal injury during high laser light dosages (2 10 J.cm about ), and long pulses (> 0.5 ms) for more efficient and effective clinical management of PWS patients. for more efficient and effective clinical management of PWS patients. To fully explore the potentials of CSC and optimize its efficiency under the most severe laser irradiation conditions that may be required for more effective treatment protocols, further studies aimed at understanding the thermodynamics and heat transfer processes associated with CSC are required. The specific objectives of this proposal are to: ( I ) develop mathematical models to predict skin internal temperature distributions following cryogen spurt termination by solving an inverse heat transfer formulation with a one dimensional inversion algorithm and subsequent, computation of temperature profiles in response to high incident light dosages and long laser pulses when specifically utilized in conjunction with CSC; (2) utilize various cryogen delivery s' about'ems to produce a wide range of droplet size, and study the subsequent thermal response of the substrate (i.e., an in-vitro epoxy resin model, and ex-vivo human skin samples, (3) conduct CSC and laser irradiation of ex-vivo human skin samples to determine optimum cooling parameters for epidermal protection under high incident light dosages and long pulse durations, and validate results of the mathematical modeling; (4) perform in-vivo experiments using the chicken comb animal model to investigate the utility of CSC in protecting the superficial tissue layers while allowing laser induced photocoagulation of dermal blood vessels; (5) utilize the information obtained from the aforementioned thermodynamic studies at theoretical, in-vitro, ex-vivo, and in-vivo animal model levels in conjunction with one-dimensional inversion of pulsed photothermal radiometry (PPTR) data to "recommend" optimum CSC and laser irradiation parameters for a specific lesion and skin type; and (6) Conduct clinical studies in PWS patients to evaluate the effectiveness of CSC in conjunction with laser irradiation using high incident dosage, longer pulses and wavelengths, and correlate the therapeutic outcome with the "recommended CSC and irradiation parameters."

描述（来自申请人摘要的逐字）： 多血管皮肤畸形，如葡萄酒红细胞染色（PWS）病变， 皮下靶血管的基于或选择性光凝固 而不会引起对表层皮肤的热损伤。校长 研究人员及其合作者对发展感兴趣， 优化冷冻剂喷雾冷却（CSC）以保护表面皮肤 层在高激光剂量期间免受热损伤（2 10 J.cm about）， 和长脉冲（> 0.5 ms），用于更高效和有效的临床管理 PWS患者更有效的PWS临床管理 患者充分挖掘CSC的潜力，优化其效率 在可能需要的最严格的激光照射条件下， 更有效的治疗方案，进一步的研究旨在了解 需要与CSC相关的热力学和传热过程。 本建议的具体目标是：（一）发展数学 预测皮肤内部温度分布的模型 通过用一个解逆传热公式来终止喷射 三维反演算法和随后的温度计算 响应于高入射光剂量和长激光脉冲的曲线， 专门与CSC结合使用;（2）使用各种冷冻剂 交付s'关于'ems产生广泛的液滴大小，并研究 衬底的后续热响应（即，体外环氧树脂 模型和离体人皮肤样品，（3）进行CSC和激光照射， 离体人皮肤样品，以确定用于 高入射光剂量和长脉冲下的表皮保护 持续时间，并验证数学建模的结果;（4）执行 使用鸡冠动物模型的体内实验来研究 CSC在保护浅表组织层同时允许激光 诱导皮肤血管光凝;（5）利用信息 从上述理论上的热力学研究中获得， 体外、离体和体内动物模型水平结合 脉冲光热辐射测量（PPTR）数据的一维反演， “推荐”特定病变的最佳CSC和激光照射参数 和皮肤类型;以及（6）在PWS患者中进行临床研究，以评估 CSC与高入射激光辐照联合作用有效性 剂量、更长的脉冲和波长，并将治疗结果 与“推荐的CSC和辐照参数。"