SPATIALLY SELECTIVE COAGULATION OF HYPERVASCULAR LESIONS

描述(逐字摘自申请者摘要)：激光治疗 血管增多的皮肤畸形如鲜红斑点(PWS)病变 表面下靶向血管的基础或选择性光凝 不会对浅层皮肤造成热损伤。校长 调查员和他的合作者对开发和 低温喷雾降温(CSC)保护浅表皮肤的优化 在高激光剂量(约210 J·cm)时热损伤的层数， 和长脉冲(&gt；0.5ms)，以实现更高效和有效的临床管理 在PWS患者中。以更有效率及更有效地进行临床治疗 病人。充分挖掘CSC潜力，优化CSC效益 在最严重的激光照射条件下，可能需要 更有效的治疗方案，进一步研究旨在了解 需要与CSC相关的热力学和热传递过程。 这项建议的具体目标是：(1)发展数学 用于预测低温后皮肤内部温度分布的模型 用1解逆传热方程解喷雾终止 量纲反演算法及后续的温度计算 高入射光剂量和长激光脉冲时的响应曲线 专门与CSC结合使用；(2)使用各种冷冻剂 交付S《关于生产各种尺寸的液滴》，并研究了 基材(即，体外环氧树脂)的后续热响应 模型和体外人皮肤标本，(3)进行CSC和激光照射 用体外人体皮肤样本确定最佳冷却参数 高入射光剂量和长脉冲下的表皮保护 持续时间，并验证数学建模的结果；(4)执行 用鸡冠动物模型进行体内实验研究 CSC在激光照射下保护浅层组织层的应用 诱导真皮血管光凝；(5)利用信息 从理论上的上述热力学研究中获得， 体外、体外和体内动物模型水平与 脉冲光热辐射测量(PPTR)数据的一维反演 为特定病变“推荐”最佳CSC和激光照射参数 和皮肤类型；以及(6)对PWS患者进行临床研究，以评估 CSC联合高强度激光照射的疗效观察 剂量、更长的脉冲和波长，并与治疗结果相关 具有“推荐的CSC和辐照参数”。

DESCRIPTION (Verbatim from Applicant's Abstract): Laser treatment of hypervascular cutaneous malformations such as port wine stain (PWS) lesion is based or selective photocoagulation of subsurface targeted blood vessels without inducing thermal damage to the superficial skin layers. The principal investigator and his collaborators are interested in development and optimization of cryogen spray cooling (CSC) to protect the superficial skin layers from thermal injury during high laser light dosages (2 10 J.cm about ), and long pulses (> 0.5 ms) for more efficient and effective clinical management of PWS patients. for more efficient and effective clinical management of PWS patients. To fully explore the potentials of CSC and optimize its efficiency under the most severe laser irradiation conditions that may be required for more effective treatment protocols, further studies aimed at understanding the thermodynamics and heat transfer processes associated with CSC are required. The specific objectives of this proposal are to: ( I ) develop mathematical models to predict skin internal temperature distributions following cryogen spurt termination by solving an inverse heat transfer formulation with a one dimensional inversion algorithm and subsequent, computation of temperature profiles in response to high incident light dosages and long laser pulses when specifically utilized in conjunction with CSC; (2) utilize various cryogen delivery s' about'ems to produce a wide range of droplet size, and study the subsequent thermal response of the substrate (i.e., an in-vitro epoxy resin model, and ex-vivo human skin samples, (3) conduct CSC and laser irradiation of ex-vivo human skin samples to determine optimum cooling parameters for epidermal protection under high incident light dosages and long pulse durations, and validate results of the mathematical modeling; (4) perform in-vivo experiments using the chicken comb animal model to investigate the utility of CSC in protecting the superficial tissue layers while allowing laser induced photocoagulation of dermal blood vessels; (5) utilize the information obtained from the aforementioned thermodynamic studies at theoretical, in-vitro, ex-vivo, and in-vivo animal model levels in conjunction with one-dimensional inversion of pulsed photothermal radiometry (PPTR) data to "recommend" optimum CSC and laser irradiation parameters for a specific lesion and skin type; and (6) Conduct clinical studies in PWS patients to evaluate the effectiveness of CSC in conjunction with laser irradiation using high incident dosage, longer pulses and wavelengths, and correlate the therapeutic outcome with the "recommended CSC and irradiation parameters."