ONCOGENIC DETERMINANTS OF THE EMERGING ALV-J RETROVIRUS

新兴 ALV-J 逆转录病毒的致癌决定因素

• 批准号: 6497517
• 负责人: KATHLEEN F CONKLIN
• 金额: $ 23.75万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497517
• 关键词: avian leukosis virus; biochemical evolution; chickens; gene expression; genetic library; genetic polymorphism; genetic recombination; northern blottings; nucleic acid probes; nucleic acid sequence; viral carcinogenesis; virus genetics; virus infection mechanism

DESCRIPTION: Avian leukemia virus-J (ALV-J) is a new non-acute transforming retrovirus that exhibits several biologically interesting features. First, ALV-J induces myeloid leukemias whereas most other ALV's induce primarily B cell lymphomas. Second, it has an envelope gene that specifies a novel subgroup (J). Third, different isolates of the virus show a high degree of genetic diversity in the env gene. Fourth, the ALV-J genome shows a complex relationship to previously described avian viruses and appears to have arisen by one or more recombination events between endogenous and exogenous viruses. Experiments in this proposal are designed to investigate several biological properties of ALV-J. Endogenous ALV-J env-related sequences have been identified that are not highly related to other viruses. Experiments are proposed (1) to determine whether evidence supports the hypothesis that these endogenous sequences contribute to the genetic diversity of infecting ALV-J virus and (2) to determine whether the presence and/or expression of the endogenous ALV-J env related sequences are associated with either an increased or decreased susceptibility to ALV-J induced malignancies. This suggestion is based on studies with murine, feline, and avian viruses that demonstrate that endogenous virus sequences can either limit or promote virus-induced disease by distinct and interesting mechanisms. Oncogenesis studies will also be conducted to investigate the unusual disease specificity of ALV-J. Two regions of ALV-J that are clearly distinct from other ALVs are the LTR and the env gene. Therefore, to determine whether either or both of these regions are important for induction of myeloid tumors by ALV-J, chimeric viruses will be generated between ALV-J and a traditional, B-lymphoma inducing strain of ALV that individually exchange the env and LTR regions and subsequently tested for oncogenicity in vivo. In addition, ALV-J induced tumors will be collected and the common integration site identified.

禽白血病病毒j （ALV-J）是一种新的非急性转化病毒

项目成果

Genome structure and expression of the ev/J family of avian endogenous viruses.

禽内源病毒ev/J家族的基因组结构和表达。

• DOI: 10.1128/jvi.73.7.5345-5355.1999
• 发表时间: 1999
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Ruis,BL;Benson,SJ;Conklin,KF
• 通讯作者: Conklin,KF