REGULATION OF GLYCEROPHOSPHOINOSITOL TRANSPORT IN YEAST

酵母中甘油磷酸肌醇运输的调控

• 批准号: 6498698
• 负责人: JANA L VOGT
• 金额: $ 21.33万
• 依托单位: DUQUESNE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6498698
• 关键词: N acylation; Saccharomyces cerevisiae; biological signal transduction; carbohydrate transport; choline; cytogenetics; fungal genetics; gene induction /repression; gene mutation; genetic promoter element; glycerophosphates; inositol; inositol phosphates; intracellular transport; microorganism metabolism; permease; phosphates; phosphatidylinositols; phospholipids; phosphorylation; posttranslational modifications; protein structure function; radiotracer; reporter genes

DESCRIPTION (Applicant's abstract): The phospholipase-mediated deacylation of phosphatidylinositol (P1) to produce extracellular glycerophosphoinositol (GroPIns) is a major catabolic pathway in S. cerevisiae cells grown in inositolcontaining medium. In times of inositol limitation, GroPlns is transported back into the cell in an energy requiring process mediated by the product of the GIT1 gene. This novel membrane permease has a number of interesting features that make it worthy of intensive study. Gitip is the first GroPlns transporter to be characterized in any organism, and there is evidence for the existence of such a transporter in higher eukaryotes. The GroPlns transported through Gitlp is used for the synthesis of PT and its inositol-containing derivatives, many of which play important roles in signal transduction. Thus, the activity of Gitip may impinge upon a number of important cellular events. In addition, evidence suggesting that the transport of GroPlns through Gitip is regulated by both phospholipid metabolism and phosphate metabolism raises the prospect of characterizing the regulatory networks linking these two fundamental aspects of cellular metabolism by means of the study of Gitip. Finally, the unique chromosomal location of GIT1 provides an opportunity to study the role of transcriptional silencing in regulating GIT1 expression. The Specific Aims of this proposal are to examine the regulation of GroPlns transport through Gitip at the transcriptional and posttranslational levels by 1) phospholipid metabolism, 2) the Unfolded Protein Response Pathway, 3) phosphate metabolism ,and 4) gene location.

描述（申请人的摘要）：磷脂酶介导的脱酰作用 磷脂酰肌醇 (P1) 产生细胞外甘油磷酸肌醇 (GroPINs) 是酿酒酵母细胞的主要分解代谢途径 含有肌醇的培养基。在肌醇限制时期，GroPlns 是 在一个需要能量的过程中被运回细胞，该过程由 GIT1基因的产物。这种新型的膜渗透性具有许多 有趣的功能使其值得深入研究。吉普是第一个 GroPlns 转运蛋白在任何生物体中都具有特征，并且有证据 高等真核生物中存在这种转运蛋白。格罗普林斯 通过Gitlp传输用于合成PT及其 含有肌醇的衍生物，其中许多在信号中发挥重要作用 转导。因此，Gitip 的活动可能会影响许多 重要的细胞事件。此外，有证据表明，运输 GroPlns 通过 Gitip 的表达受到磷脂代谢和 磷酸盐代谢提高了表征监管的前景 通过以下方式将细胞代谢的这两个基本方面联系起来的网络 Gitip 的研究。最后，GIT1独特的染色体位置 提供了研究转录沉默的作用的机会 调节 GIT1 表达。该提案的具体目标是审查 GroPlns 通过 Gitip 在转录和转录过程中的运输的调节 翻译后水平通过 1) 磷脂代谢，2) 未折叠蛋白 响应途径，3) 磷酸盐代谢，4) 基因定位。