MAMMALIAN CELL CYCLE CHECKPOINT GENES

哺乳动物细胞周期检查点基因

• 批准号: 6489116
• 负责人: LEI LI
• 金额: $ 12.12万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-15 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6489116
• 关键词: DNA damage; DNA repair; DNA replication; Saccharomyces cerevisiae; Schizosaccharomyces pombe; animal genetic material tag; antibody formation; antisense nucleic acid; cell cycle; cell line; electroporation; fluorescent in situ hybridization; gene targeting; genetic mapping; human genetic material tag; laboratory mouse; molecular cloning; neoplasm /cancer genetics; tumor suppressor genes; yeast two hybrid system

DESCRIPTION: Cell cycle checkpoints arrest the cell division cycle at specific stages so DNA repair pathways can restore the integrity of the genome. Defects in checkpoint pathways can cause genomic instability and lead to cancer. A human cDNA, called hsrad17, has been cloned as the human homologue of fission yeast rad17, an essential component of the yeast S. pombe DNA damage/replication block checkpoint pathway. The objective of this proposal is to characterize hsrad17 in terms of its checkpoint function in mammalian cells, as well as its possible role as a tumor suppressor. Antisense technology and the introduction of antibodies will be used to deplete hsrad17 protein, and its role in checkpoint control will be tested. The hsrad17 locus will be mapped to a human chromosome. LOH and possible hsrad17 mutations in cancer will be investigated. Genetic screens will be used to isolate proteins that interact with the hsrad17 gene product to determine its function and the cell cycle pathways in which it is involved.

描述：细胞周期检查点在

项目成果

Human Rad17 is phosphorylated upon DNA damage and also overexpressed in primary non-small cell lung cancer tissues.

• 发表时间: 2001-10
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: X. Wang;L. Wang;M. Callister;J. Putnam;L. Mao;L. Li