Engineering Acetobacter using Synthetic Biology to improve its industrial properties

细菌纤维素是一种超纯碳水化合物聚合物，具有无数有用的特性。由于其纯度，它具有高结晶结构和高拉伸强度，除了持水潜力。因此，它是生物技术行业非常感兴趣的，因为它提供了一种独特的材料，可用于从过滤膜到医疗设备的一系列应用。它主要是通过化学或酶处理来实现功能化，但是我们开始使用合成生物学来实现细菌纤维素的功能化，利用KTK金门工具包来设计雷提库菌的代谢，雷提库菌是一种来自醋酸杆菌家族的生物。在生长过程中形成纤维素膜作为其生物膜。我们的目标是设计雷蒂库菌的代谢来控制纤维素的生产。此外，我们希望功能化膜，使其具有可用于许多应用的特性。我们还努力与雷氏酵母和酵母建立群落，通过分泌蛋白质（如纤维素结合结构域（CBDs）杂交蛋白）来实现膜的功能化，使其附着在纤维素上，同时保持初始蛋白质的能力。不仅改变了纤维素膜的形态，而且使其具有反应功能。为纤维素提供新的性能、抗拉强度和结晶度的工程是该项目的核心。

Bacterial cellulose is an ultra-pure carbohydrate polymer which has a myriad of useful properties. Due to its purity it has a highly crystalline structure and a high tensile strength, in addition to water holding potential. Therefore, it is of great interest to the biotechnology industry as it provides a unique material that can be used in a range of applications from filtration membranes to medical devices. It's is mainly functionalised using chemical or enzymatic processed however we set out to functionalise bacterial cellulose using Synthetic Biology to engineer the metabolism of K. rhaeticus, an organism from the Acetobacter family, using the KTK Golden Gate toolkit. During the growth of K. rhaeticus it forms a cellulose pellicle as its biofilm. We aim to engineer the metabolism of K. rhaeticus to control cellulose production. In addition, we wish to functionalise the pellicle giving it properties that can be used in many applications. We also endeavour to set up communities with K. rhaeticus and yeast to functionalise the pellicle by secreting proteins such as cellulose-binding-domains (CBDs) hybrid proteins to attach onto the cellulose while maintain the initial proteins abilities. Not only altering the morphology of the cellulose pellicle but also giving it a reactive function. The engineering of cellulose to provide it new abilities, tensile strength and crystallinity is the core of this project.