Striatum/Amygdalostriatal Area

描述：（申请人摘要）腹侧（边缘相关）纹状体 介导动机行为，被认为是重要的解剖基质 精神病杏仁核是一个突出的边缘结构， 刺激他们的情感意义。腹侧纹状体可以 定义是基于来自调节情绪处理的结构的输入， 杏仁核;与杏仁核相连的眶内侧前额区，以及 背侧层多巴胺（DA）神经元。腹侧纹状体的“壳”是一个独特的 从这些结构获得有限投入的次区域， 特征为1.相对低的钙结合蛋白-Dk28（CaBP）染色，2.多 具有不同分布的F物质的细胞（“界面”）岛， 脑啡肽、乙酰胆碱酯酶（AchE）、酪氨酸羟化酶（TH）和D1 mRNA，3.杏仁核传入神经和细胞之间精确重叠的区域 与特定发射器相关联的集群，4.紧密重叠区域 杏仁核和皮质传入神经之间的联系 我们假设尾腹侧纹状体和邻近的杏仁核纹状体 区域（Astr）是腹侧'边缘'纹状体的一个特殊部分， 颞叶的输入，基于类似的标准。颞叶介导 听觉和视觉处理，与杏仁核相互联系，是一个 精神分裂症的异常部位。我们的初步数据显示， 腹侧纹状体、尾腹侧纹状体/Astr区：1.接收 来自调节情绪的大脑结构的传入：杏仁核，特定区域 大脑皮层与杏仁核和多巴胺能神经元相连。2.接收来自 DA神经元“背侧层”的尾侧成分3.有一个钙结合蛋白缺乏的区域， 以及多个细胞岛， F物质、脑啡肽、AchE、TH和D1 mRNA，与在 壳 这些研究的第一个目标是确定尾腹侧 纹状体/Astr区是腹侧纹状体的一部分， 细胞结构和组织化学特征。我们的第二个目标是检查 杏仁核投射到含有物质的细胞簇的假说 P和D1受体mRNA在尾腹侧纹状体/Astr区，因此， 能够激活这些递质/受体特异性通路。我们 第三个目标是确定杏仁核和颞叶皮层的输入是否 在尾腹侧纹状体/Astr区会聚。这些研究的结果 将提供一个解剖学框架， 重要的听觉和视觉信息在纹状体中处理。

DESCRIPTION: (Applicant's abstract) The ventral ('limbic-related) striatum mediates motivated behavior, and is considered an important anatomic Substrate of psychosis. The amygdala, a prominent limbic structure, links complex sensory stimuli to their emotional significance. One way the ventral striatum can be defined is based on inputs from structuresthat mediate emotional processing the amygdala; orbitomedial prefrontal regions linked to the amygdala, and the dorsal tier dopamine (DA) neurons. The ventral striatal 'shell' is a unique subregion that receives restricted inputs from these structures, and is charactenzed by 1. Relatively low calbindin-Dk28 (CaBP) staining, 2. Multiple cellular ('interface') islands with differential distributions of substance F, enkephalin, acetyicholinesterase (AchE), tyrosine hydroxylase (TH), and D1 mRNA, 3. Regions of precise overlap between amygdaloid afferents and cell clusters associated with specific transmitters, 4. Regions of tight overlap between amygdaloid and cortical afferents. We hypothesize that the caudoventral striatum and adjacent amygdalostriatal area (Astr) is a special part of the ventral 'limbic' striatum that is devoted to temporal lobe inputs, based on similar criteria. The temporal lobes mediate auditory and visual processing, are interconnected with the amygdala, and are a site of abnormalities in schizophrenia. Our preliminary data suggest that, like the ventral striatum, the caudoventral striatum/Astr area: 1. Receives afferents from brain structures mediating emotion: amygdala, specific regions of cortex linked to the amygdala, and the DA neurons. 2. Receives input from a caudal component of the 'dorsal tier' of DA neurons 3. Has a CaBP-poor region, and multiple cellular islands associated with varying distributions of substance F, enkephalin, AchE, TH, and D1 mRNA, similar to that found in the shell. The first goal of these studies is to determine whether the caudoventral striatum/Astr area is part of the ventral striatum based on connectional, cytoarchitectural, and histochemical features. Our second aim is to examine the hypothesis that the amygdala projects to cellular clusters containing substance P and Dl receptor mRNA in the caudoventral striatal/Astr area, and is therfore in a position to activate these transmitter/receptor specific pathways. Our third aim is to determine whether amygdaloid and temporal cortical inputs converge in the caudoventral striatum/Astr area. The results of these studies will provide an anatomic framework for understanding how emotionally significant auditory and visual information is processed in the striatum.