STUDIES OF REGIONAL FAT DISTRIBUTION AND ENERGY BALANCE

区域脂肪分布与能量平衡的研究

• 批准号: 6498095
• 负责人: DAVID S WEIGLE
• 金额: $ 9.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6498095
• 关键词: AIDS therapy; HIV infections; adipose tissue; bioenergetics; body composition; clinical research; cortisol; dietary carbohydrates; dietary lipid; gene expression; hormone receptor; human subject; leptin; lipoprotein lipase; nutrition related tag; obesity; peroxisome proliferator activated receptor; weight control

The studies in this application are designed to better understand the regulation of body fat mass and the mechanisms determining body fat distribution in humans. Three Specific Aims are proposed: 1. To determine whether experimentally-induced or spontaneous variations in plasma leptin levels predict subsequent changes in body composition. The protocols employed will also elucidate the mechanism by which high carbohydrate diets induce satiety and weight loss, and the mechanism of involuntary weight loss in the elderly. 2. To examine the relationships among central body fat redistribution, changes in adipogenic gene expression, and atherogenic changes in plasma lipids and insulin sensitivity. The model for this work will be the body fat redistribution that occurs in subjects receiving aggressive antiretroviral therapy for HIV infection. 3. To determine whether changes in uncoupling protein (UCP) gene expression act to attenuate diet-induced changes in body fat mass. This study will use the technology developed for Specific Aim 2 to ascertain whether muscle and fat UCP expression change in parallel with energy expenditure following experimental weight gain and weight loss in human subjects. The applicant has successfully trained several young physician investigators in patient-oriented and basic research, and a plan to continue doing so is presented.

本申请中的研究旨在更好地了解人体脂肪质量的调节和决定人体脂肪分布的机制。提出了三个具体的目标：1.确定血浆瘦素水平的实验诱导或自发变化是否可以预测随后的身体成分变化。所采用的方案还将阐明高碳水化合物饮食导致饱腹感和体重减轻的机制，以及老年人非自愿减肥的机制。2.探讨中心体脂再分布、成脂基因表达变化与血脂、胰岛素敏感性等致动脉粥样硬化的关系。这项工作的模型将是在接受艾滋病毒感染的积极抗逆转录病毒治疗的受试者中发生的体脂重新分配。3.确定解偶联蛋白(UCP)基因表达的变化是否在减轻饮食诱导的体脂含量变化中起作用。这项研究将使用为特定目的2开发的技术来确定人体受试者在实验体重增加和减轻后，肌肉和脂肪UCP的表达是否与能量消耗同步变化。申请人已经成功地培训了几名年轻的内科研究人员，从事以患者为中心的基础研究，并提出了继续这样做的计划。