Adaptation to anaerobiosis in H influenzae pathogenesis

描述（由申请人提供）：流感嗜血杆菌是一种常见的流感嗜血杆菌。 上呼吸道的无症状定植者，但也会引起一系列 侵袭性疾病，如中耳炎，一种中耳感染，可产生 儿童的急性疼痛、痛苦以及暂时或永久性听力损失。的 中耳中存在流感嗜血杆菌专性厌氧菌种 中耳炎患者的液体和鼻咽部检查表明流感嗜血杆菌 体内遇到低氧条件。流感嗜血杆菌具有 在其他几种细菌病原体中发现了氧化还原反应系统。的 这些氧化还原反应系统参与改变整体基因 将通过以下方式研究对缺氧反应的表达 表征硝酸盐和亚硝酸盐还原酶的表达谱。 初步数据表明硝酸盐和亚硝酸盐还原酶在幽门螺杆菌中表达。 流感杆菌由环境信号介导，例如底物可用性 和环境氧气水平。此外，体外表征 硝酸盐和亚硝酸盐还原酶表达谱允许开发 还原酶编码基因作为幽门螺杆菌生理状态的报告基因。 幼鼠感染期间的流感病毒。因此，本研究的重要性 不仅是为了深入了解氧化还原传感的调节过程 流感嗜血杆菌，还可以表征其生理状态 宿主生态位内的细菌。

DESCRIPTION (provided by applicant): Haemophilus influenzae is a common asymptomatic colonizer of the upper respiratory tract, yet also causes a range of invasive diseases such as otitis media, a middle ear infection that produces acute pain, distress and temporary or permanent hearing loss in children. The presence of obligate anaerobic species with H. influenzae in the middle ear fluid and the nasopharynx of otitis media patients indicates that H. influenzae encounters low oxygen conditions in vivo. H. influenzae possesses redox-responsive systems found in several other bacterial pathogens. The participation of these redox-responsive systems in altering global gene expression in response to oxygen deprivation will be investigated by characterizing the expression profiles of the nitrate and nitrite reductases. Preliminary data indicate that nitrate and nitrite reductase expression in H. influenzae is mediated by environmental signals, such as substrate availability and environmental oxygen levels. Additionally, the in vitro characterization of the nitrate and nitrite reductase expression profiles permits the development of the reductase-encoding genes as reporters for the physiological state of H. influenzae during infant rat infection. Therefore, the importance of this study is to not only yield insight into the regulatory processes of redox sensing in H. influenzae, but also to characterize the physiological state of this bacterium within host niches.