DIETARY CAROTENOIDS - LIPOPROTEIN/CELL INTERACTIONS

膳食类胡萝卜素 - 脂蛋白/细胞相互作用

• 批准号: 6435490
• 负责人: EARL Howard HARRISON
• 金额: $ 17.5万
• 依托单位: U.S. DEPARTMENT OF AGRICULTURE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6435490
• 关键词: antioxidants; atherosclerosis; blood lipoprotein; carotenoids; cell line; cholesterol; chylomicrons; clinical research; dietary constituent; gastrointestinal absorption /transport; gene expression; human subject; intestines; lipid transport; macrophage; monocyte; nutrient bioavailability; nutrition related tag; phenotype; retinoids; stable isotope; sterols; tissue /cell culture; vitamin metabolism

Humans obtain carotenoids in the diet from fruits and vegetables. The role of carotenoids as antioxidants has been the focus of our work on lipoprotein oxidation. While it is clear that lipoproteins are the main vehicles for transport of carotenoids, little is known about mechanisms of intestinal absorption of carotenoids. We now seek to define the mechanism(s) of intestinal absorption of carotenoids and their distribution into blood cells. We focus on the interaction of carotenoids and the human monocyte/macrophage since this interaction may play a role in the development of atherosclerosis. Specific aims are: 1. Mechanisms of intestinal absorption of carotenoids and their incorporation into chylomicrons in CACO-2 cells in culture. We will test the hypothesis that other fat-soluble nutrients inhibit the incorporation of beta-carotene into chylomicrons. We will test the hypothesis that SRB1, CD-36, FAT, and other putative lipid transporters mediate the uptake of beta-carotene into the cell. We will test the hypothesis the increasing the expression of beta-carotene 15,15' dioxygenase increases the total flux of beta-carotene through the cell. 2. Intestinal absorption of beta-carotene in humans. We will use stable isotope methodology to study the mechanisms of the intestinal absorption of beta- carotene in humans. The general question under investigation is whether there are common mechanisms for the absorption of various fat-soluble dietary nutrients. We will test the hypothesis that the efficiency of intestinal absorption of cholesterol is positively correlated with the efficiency of the intestinal absorption of beta-carotene. We will test the hypothesis that beta-carotene is converted to vitamin A solely in the intestine. We will quantify the conversion of beta-carotene to vitamin A to test the hypothesis that increased absorption of beta-carotene is associated with increased conversion to vitamin A. 3. Modulation of macrophage function by dietary carotenoids. We will use the U937 cell line as well as human peripheral blood monocyte- macrophages to examine the partitioning of carotenoids into blood-borne cells and the effects of carotenoid enrichment on monocyte-macrophage function. We will test the hypothesis that plasma carotenoids partition into various blood cells as well as into plasma lipoproteins. We will test the hypothesis that carotenoid enrichment alters monocyte-macrophage phenotype, either enhancing or inhibiting potentially pro-atherosclerotic aspects of cell function including response to chemotactic stimuli, adhesion to endothelial cells, differentiation into macrophages, ability to oxidize LDL, and ability to form foam cells.

人类从水果和蔬菜中获取类胡萝卜素。 类胡萝卜素作为抗氧化剂的作用一直是我们研究脂蛋白氧化的重点。 虽然脂蛋白是运输类胡萝卜素的主要载体，但对类胡萝卜素的肠道吸收机制知之甚少。 我们现在试图确定类胡萝卜素的肠道吸收机制及其分布到血细胞中。 我们专注于类胡萝卜素和人单核细胞/巨噬细胞的相互作用，因为这种相互作用可能在动脉粥样硬化的发展中发挥作用。 具体目标是：1。类胡萝卜素的肠道吸收机制及其与培养的CACO-2细胞乳糜微粒的结合。 我们将检验其他脂溶性营养素抑制β-胡萝卜素掺入乳糜微粒的假设。 我们将测试的假设，SRB 1，CD-36，脂肪，和其他假定的脂质转运介导的β-胡萝卜素的摄取到细胞。我们将检验增加β-胡萝卜素15，15 '双加氧酶的表达增加β-胡萝卜素通过细胞的总通量的假设。2. 人体对β-胡萝卜素的肠道吸收。 我们将使用稳定同位素方法学来研究人体肠道吸收β-胡萝卜素的机制。 正在调查的一般问题是，是否有各种脂溶性膳食营养素的吸收的共同机制。 我们将检验胆固醇的肠道吸收效率与β-胡萝卜素的肠道吸收效率正相关的假设。 我们将测试β-胡萝卜素仅在肠道中转化为维生素A的假设。我们将量化β-胡萝卜素向维生素A的转化，以检验β-胡萝卜素吸收增加与维生素A转化增加相关的假设。 3.膳食类胡萝卜素对巨噬细胞功能的调节。 我们将使用U937细胞系以及人外周血单核细胞-巨噬细胞来检查类胡萝卜素向血液传播细胞的分配以及类胡萝卜素富集对单核细胞-巨噬细胞功能的影响。 我们将检验血浆类胡萝卜素分配到各种血细胞以及血浆脂蛋白的假设。 我们将测试的假设，类胡萝卜素富集改变单核细胞-巨噬细胞的表型，无论是增强或抑制潜在的促动脉粥样硬化方面的细胞功能，包括响应趋化刺激，粘附内皮细胞，分化成巨噬细胞，氧化LDL的能力，并形成泡沫细胞的能力。