Sterilisable, biocompatible, immersible, spectroscopic planar imaging system

可消毒、生物相容性、浸入式光谱平面成像系统

• 批准号: 2072626
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2072626
• 关键词: Sterilisable; biocompatible; immersible; spectroscopic; planar

Current bioreactors use offline testing to analyse the quality and status of their samples. This is a costly and wasteful process, and limits the frequency at which the bioreactor contents can be tested. It is proposed that a system could be developed using a sterilisable monolithic image transfer system to allow continuous measurement of the reactor contents, without damaging or removing cells. To do this, a dual system would be used; it would be composed of a high quality imaging system capable of spatial resolution to 10 micro m, coupled with a Raman system capable of measuring the concentration of key nutrients within the medium. The imaging system will be used to perform cell counting, in order to calculate the cell concentration within the medium. One of the key concerns for such a technique is the damage that might be caused by injecting light into the cells. Part of this body of research will therefore revolve around measuring and analysing the causes of phototoxicity and photomutagenicity in human stem cells. This will feed into the development of the prototype machine, affecting the illumination intensity and wavelength.The aim of the project would be to test the viability of a dual imaging/Raman system for use in the processing of human stem cells, to publish data regarding the efficacy of dual imaging/Raman systems, and to feed into UCL's existing bioengineering facilities. The project would also investigate the problems associated with using optical measurement systems on sensitive biological matter, such as phototoxicity and photomutagenicity, and what precautions can be taken to negate or mitigate them.The benefit expected of the proposed system would be the ability to measure nutrient concentration over scales of less than a minute, whilst simultaneously measuring the cell concentration. Due to the nature of the monolithic image transfer plate, the Raman system could potentially be extended to allow for Raman imaging that could provide sub-second images of nutrient movement around cells, opening up the potential for research into the uptake rate of various nutrients during cellular processes.In addition to the benefits brought by the system, researching phototoxicity and its effects should open up the possibility of other optical methods for use in bioreactors. This might not be limited to sensing technologies, but also intentional cell mutation using light, or the manipulation of cells using light.The project would take the form of designing and building a prototype system, before verifying and demonstrating its efficacy experimentally. Expert opinions from bioengineers within the department will be sought to find potential improvements that would make the device more useful in the development of industrial and hospital medicine.This technology would align with several EPSRC Healthcare challenges. In particular, it addresses Developing Future Therapies, as it would greatly improve the processes used in developing future therapies, by allowing online measurement of the reactor. Cell therapies are minimally invasive, and could remove the need for damaging surgeries to remove, for example, cancerous tumours, Which would align with the Frontiers of Physical Intervention challenge. It develops the tools vital to the quantitative analysis of cellular manufacturing techniques, addressing the Manufacturing priority New Industrial Systems.

目前的生物反应器使用离线测试来分析样品的质量和状态。这是一个昂贵和浪费的过程，并且限制了生物反应器内容物可以测试的频率。有人建议，一个系统可以开发使用可消毒的单片图像传输系统，允许连续测量反应器内容物，而不损坏或移除细胞。为此，将采用双重制度；它将由一个空间分辨率可达10微米的高质量成像系统和一个能够测量培养基中关键营养物质浓度的拉曼系统组成。成像系统将用于细胞计数，以计算培养基中的细胞浓度。这种技术的关键问题之一是将光注入细胞可能造成的损伤。因此，该研究机构的一部分将围绕测量和分析人类干细胞的光毒性和光致突变性的原因展开。这将馈送到样机的研制中，影响光照强度和波长。该项目的目的是测试用于处理人类干细胞的双成像/拉曼系统的可行性，发布关于双成像/拉曼系统功效的数据，并为伦敦大学学院现有的生物工程设施提供数据。该项目还将调查在敏感生物物质上使用光学测量系统所涉及的问题，例如光毒性和光致变色性，以及可以采取哪些预防措施来消除或减轻这些问题。预计该系统的好处是能够在不到一分钟的时间内测量营养物质浓度，同时测量细胞浓度。由于单片图像传输板的特性，拉曼系统可能被扩展到允许拉曼成像，可以提供细胞周围营养物质运动的亚秒图像，为研究细胞过程中各种营养物质的摄取速率开辟了潜力。除了该系统带来的好处外，研究光毒性及其效应应该为生物反应器中使用其他光学方法开辟可能性。这可能不仅限于传感技术，还包括利用光进行有意的细胞突变，或利用光操纵细胞。该项目将采用设计和建造原型系统的形式，然后通过实验验证和证明其有效性。将寻求该部门生物工程师的专家意见，以找到可能的改进，使该设备在工业和医院医学的发展中更有用。这项技术将与EPSRC医疗保健面临的几个挑战保持一致。特别是，它解决了开发未来疗法，因为它将极大地改进用于开发未来疗法的过程，通过允许对反应器进行在线测量。细胞疗法具有最小的侵入性，并且可以消除切除癌症肿瘤等破坏性手术的需要，这将与物理干预前沿挑战相一致。它开发了对细胞制造技术的定量分析至关重要的工具，解决了制造优先级的新工业系统。