Effect of O2 Stress and Aging on Single Myocyte Function

氧气应激和衰老对单肌细胞功能的影响

• 批准号: 6509995
• 负责人: KEVIN M KELLEY
• 金额: $ 1.57万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6509995
• 关键词: aging; calcium; exercise; fatigue; free radical oxygen; homeostasis; hypoxia; laboratory mouse; muscle cells; muscle function; oxidative stress; postdoctoral investigator; single cell analysis; striated muscles

DESCRIPTION: (provided by applicant) Skeletal muscle function declines with age. With the increase in man?s average expected life span and the increasing elderly population worldwide, scientific research is attempting to preserve the aging population?s quality of life. One theory at the forefront of explanations for the effects of aging has been the cumulative negative effects of free radical production over time. Evidence suggests that aged organisms grow increasingly vulnerable to damage from reactive oxygen species (ROS). In addition to the progressive atrophy and functional declines observed in aging skeletal muscle, common medical conditions in the elderly such as respiratory and circulatory insufficiencies can compromise the body?s ability to deliver oxygen to tissues throughout the body. These combined effects of aging and hypoxia on skeletal muscle car severely compromise functional capacity in these individuals and further hasten the loss of physical independence. I propose to use the single skeletal muscle fiber model in which experimental conditions can be tightly controlled and several intracellular factors can be examined in an intact, contracting fiber. By I studying the alterations that occur in the aged muscle?s oxidative stress and its ability to respond to exercise and hypoxia, a better understanding of how exercise prescription and drug therapy can prevent or improve muscle function with age may be achieved.

描述：（由申请人提供）骨骼肌功能下降 年龄。随着人类平均预期寿命的增加和 全世界的老年人口，科学研究正试图保护 人口老龄化的生活质量。处于解释前沿的一种理论 衰老的影响是免费的累积负面影响 随着时间的推移，激进的生产。有证据表明衰老的生物体会生长 越来越容易受到活性氧 (ROS) 的损害。在 除了在衰老过程中观察到的进行性萎缩和功能衰退之外 骨骼肌、老年人常见疾病，如呼吸系统疾病 循环不足会损害身体的输送能力 为全身组织提供氧气。衰老和这些因素的综合影响 骨骼肌缺氧会严重损害这些部位的功能能力 并进一步加速丧失身体独立性。我建议 使用单骨骼肌纤维模型，其中实验条件可以 受到严格控制，并且可以在一个过程中检查多种细胞内因子 完整的、收缩的纤维。通过我研究老年人发生的变化 肌肉的氧化应激及其对运动和缺氧的反应能力， 更好地了解运动处方和药物治疗如何预防 或者随着年龄的增长可以实现肌肉功能的改善。