Wide field imaging at depth for the biomedical sciences

生物医学领域的深度广域成像

• 批准号: 2093575
• 金额: --
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2093575
• 关键词: Wide; field; imaging; depth; biomedical

Light-sheet microscopy (LSM) is a powerful approach for fast wide field, low phototoxicity imaging. Its ultimate limit is optical penetration into the specimen. Existing approaches to overcome this challenge include illuminating the sample from multiple sides, the use of chemical clearing methods or sophisticated aberration correction. Though effective, a challenge is to develop facile methods for biomedical researchers and the emergent area of use in pathology. Recent advances, including those by our team, in high-resolution LSM have exploited advanced propagation-invariant modes of light, in particular Bessel, Airy and 'lattice' modes, for wide field of view with high resolution. This can achieve 0.000001 m resolution over 100s of micron field of view, but how may we obtain excellent spatiotemporal resolution (e.g. 100s Hz, FOV up to mm, sub diffractive imaging)? For this we can use super-resolution imaging as well. This will include structured illumination microscopy as well as other algorithmsThis PhD project will explore appropriate combinations of light sheet and super-resolved imaging for studies in biomedicine. Particular studies will include applications for histopathology for colon cancer, lung cancer and renal disease with imaging on appropriate tissue samples.

光片显微镜（LSM）是一种快速宽视场、低光毒性成像的有效方法。其极限是光穿透到试样中。克服这一挑战的现有方法包括从多个侧面照射样品，使用化学清除方法或复杂的像差校正。虽然有效，但挑战是为生物医学研究人员和病理学中的新兴应用领域开发简便的方法。最近的进展，包括我们的团队，在高分辨率LSM利用先进的光的传播不变模式，特别是贝塞尔，艾里和“晶格”模式，为高分辨率的宽视场。这可以在100秒的微米视场内实现0.000001米的分辨率，但我们如何获得优异的时空分辨率（例如100秒Hz，FOV高达mm，亚衍射成像）？为此，我们也可以使用超分辨率成像。这将包括结构照明显微镜以及其他algorithmsThis博士项目将探索光片和超分辨成像在生物医学研究的适当组合。具体的研究将包括结肠癌、肺癌和肾病的组织病理学应用，并对适当的组织样本进行成像。