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SFM VISUALIZATION OF SINGLE CHROMATIN FIBER DYNAMICS

SFM VISUALIZATION OF SINGLE CHROMATIN FIBER DYNAMICS
单染色质纤维动力学的 SFM 可视化
批准号：
2628851
负责人：
SANFORD H LEUBA
金额：
$15.02万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
项目状态：
已结题
起止时间：
2002-09-27 至 2004-08-31
来源：
https://reporter.nih.gov/project-details/2628851
关键词：
DNA directed RNA polymerase atomic force microscopy bioimaging /biomedical imaging chromatin mouse mammary tumor virus nanotechnology nucleic acid repetitive sequence nucleosomes physical model protein binding protein protein interaction solubility transcription factor

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中文摘要
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英文摘要
DESCRIPTION: (Applicant?s Description) The major goals of this project are to perform biochemical experiments at the single nucleosome or fiber level in buffer as visualized by scanning force microscopy. Experimental plans can be roughly separated into three parts: (1) Reversible compaction of individual fibers followed in buffer by SFM. Images of the same fiber in low, moderate and high salts will be used to generate models of fiber structure. A new, well-phased fiber will be created for this purpose. (2) What occurs when an elongating RNA polymerase encounters a nucleosome? Experiments are designed to ask what happens when a single RNA polymerase encounters a single nucleosome in buffer in real time. (3) How do nucleosomes in the Mouse Mammary Tumor Virus (MMTV) Long Terminal Repeat (LTR) interact with transcription factors? Are specific nucleosomes displaced or are their structures altered to allow transcription factor binding?
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Development of novel single-molecule approaches for nanoscale study of helicases
Development of novel single-molecule approaches for nanoscale study of helicases
Development of novel single-molecule approaches for nanoscale study of helicases
Development of novel single-molecule approaches for nanoscale study of helicases
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