Search for a Hypertensive Renal Failure Gene

描述：(申请人提供)：终末期肾病(ESRD)仍是 这是美国的主要健康问题，而且每年都在继续增加。 到1997年，有超过300,000名患者参加了医疗保险基金ESRD 肾脏替代治疗计划是否会降低终末期肾病的护理总费用 美国的患者每年超过150亿美元。这样做的目的是 项目是寻找一个或多个基因，使其对癌症易感 高血压性终末期肾病大鼠模型。大鼠1号染色体的一个区域以前 被鉴定为携带肾功能衰竭基因(称为RF-1)，来自 回交-已在F2杂交中复制的回交，两者均源自 小鹿冠状高血压(FHH)大鼠及其正常血压ACI亲本株 老鼠。在提议的项目中，我将使用各种不同的 最先进的分子遗传学技术旨在缩小 可能致病基因的宇宙；最终基因将是 在病株(FHH)和正常株系(ACI，Brown)中都进行了测序 挪威)，以确定可能导致 对高血压ESRD的易感性。正如人们所相信的， 人类肾脏疾病的发病和进展与 这项工作最终可能会导致对ESRD更好的理解 人类。

DESCRIPTION: (provided by applicant): End-stage renal disease (ESRD) remains a major health problem in the United States and continues to increase annually. By 1997 there were over 300,000 patients enrolled in the Medicare-fund ESRD program for renal replacement therapy, will the total cost of care for ESRD patients in the United States at over $15 billion annually. The goal of this project is to search for a gene (or genes) that confers susceptibility to hypertensive ESRD in a rat model. A region of rat chromosome 1 has previously been identified as harboring a gene for renal failure (termed Rf-1), from a backcross-that has been replicated in an F2 intercross, both derived from the Fawn Hooded Hypertensive (FHH) rat and the normotensive ACI parental strains of rat. Under the proposed project I will search for this gene using a variety of state of the art molecular genetics techniques designed to narrow down the universe of possible disease-causing genes; ultimately the gene will be sequenced in both the disease strain (FHH) and normal strains (ACI, Brown Norway) to identify molecular changes potentially responsible for the susceptibility to hypertensive ESRD. As it is believed that the mechanisms of renal disease onset and progression are the same or similar in humans as in rats, this work may ultimately lead to a better understanding of ESRD in humans.