Search for a Hypertensive Renal Failure Gene

描述：（由申请人提供）：终末期肾病（ESRD）仍然是 这是美国的主要健康问题，每年都在增加。 到1997年，有超过30万名患者参加了由医疗保险基金资助的终末期肾病 肾脏替代治疗计划，终末期肾病的总护理费用 美国的病人每年花费超过150亿美元。这个目标 项目是寻找一个基因（或基因），赋予易感性， 高血压终末期肾病大鼠模型。大鼠1号染色体上的一个区域 已被鉴定为携带肾衰竭基因（称为Rf-1），从一个 回交-已经在F2互交中复制的，两者都来自 Fawn Hooded Hypertensive（FHH）大鼠和血压正常的ACI亲本品系 大鼠在拟议的项目中，我将使用各种方法来寻找这种基因。 最先进的分子遗传学技术，旨在缩小 可能的致病基因的宇宙;最终基因将是 在疾病菌株（FHH）和正常菌株（ACI，Brown）中测序 挪威），以确定可能导致 易患高血压终末期肾病。据信， 肾脏疾病的发作和进展在人类中与在 大鼠，这项工作可能最终导致更好地了解终末期肾病， 人类

DESCRIPTION: (provided by applicant): End-stage renal disease (ESRD) remains a major health problem in the United States and continues to increase annually. By 1997 there were over 300,000 patients enrolled in the Medicare-fund ESRD program for renal replacement therapy, will the total cost of care for ESRD patients in the United States at over $15 billion annually. The goal of this project is to search for a gene (or genes) that confers susceptibility to hypertensive ESRD in a rat model. A region of rat chromosome 1 has previously been identified as harboring a gene for renal failure (termed Rf-1), from a backcross-that has been replicated in an F2 intercross, both derived from the Fawn Hooded Hypertensive (FHH) rat and the normotensive ACI parental strains of rat. Under the proposed project I will search for this gene using a variety of state of the art molecular genetics techniques designed to narrow down the universe of possible disease-causing genes; ultimately the gene will be sequenced in both the disease strain (FHH) and normal strains (ACI, Brown Norway) to identify molecular changes potentially responsible for the susceptibility to hypertensive ESRD. As it is believed that the mechanisms of renal disease onset and progression are the same or similar in humans as in rats, this work may ultimately lead to a better understanding of ESRD in humans.