Search for a Hypertensive Renal Failure Gene

寻找高血压肾衰竭基因

• 批准号: 6626222
• 负责人: Michael Ignatius Jensen-Seaman
• 金额: $ 4.64万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626222
• 关键词: gene mutation; genetic susceptibility; genetics; introns; laboratory rat; nucleic acid sequence; renal hypertension

DESCRIPTION: (provided by applicant): End-stage renal disease (ESRD) remains a major health problem in the United States and continues to increase annually. By 1997 there were over 300,000 patients enrolled in the Medicare-fund ESRD program for renal replacement therapy, will the total cost of care for ESRD patients in the United States at over $15 billion annually. The goal of this project is to search for a gene (or genes) that confers susceptibility to hypertensive ESRD in a rat model. A region of rat chromosome 1 has previously been identified as harboring a gene for renal failure (termed Rf-1), from a backcross-that has been replicated in an F2 intercross, both derived from the Fawn Hooded Hypertensive (FHH) rat and the normotensive ACI parental strains of rat. Under the proposed project I will search for this gene using a variety of state of the art molecular genetics techniques designed to narrow down the universe of possible disease-causing genes; ultimately the gene will be sequenced in both the disease strain (FHH) and normal strains (ACI, Brown Norway) to identify molecular changes potentially responsible for the susceptibility to hypertensive ESRD. As it is believed that the mechanisms of renal disease onset and progression are the same or similar in humans as in rats, this work may ultimately lead to a better understanding of ESRD in humans.

描述：（由申请人提供）：终末期肾病（ESRD）仍然是一种 是美国主要的健康问题，并且逐年增加。 到 1997 年，已有超过 300,000 名患者参加了医疗保险基金 ESRD 肾脏替代治疗计划，终末期肾病 (ESRD) 的总护理费用 美国每年的患者价值超过 150 亿美元。此举的目标 项目的目的是寻找一个（或多个）赋予易感性的基因 大鼠模型中的高血压 ESRD。大鼠 1 号染色体的一个区域先前已 被鉴定为携带肾衰竭基因（称为 Rf-1），来自 回交-已在 F2 杂交中复制，两者均源自 小鹿连帽高血压 (FHH) 大鼠和正常血压 ACI 亲本品系 鼠。在拟议的项目下，我将使用各种方法来搜索该基因 最先进的分子遗传学技术旨在缩小范围 可能致病基因的宇宙；最终该基因将是 在疾病菌株 (FHH) 和正常菌株 (ACI、Brown 挪威）以确定可能导致的分子变化 高血压 ESRD 易感性。正如人们所相信的，其机制 人类肾脏疾病的发病和进展与人类相同或相似 大鼠，这项工作最终可能会导致人们更好地了解 ESRD 人类。