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AFM Sensors to Detect Biological Warfare Agents

用于检测生物战剂的 AFM 传感器

基本信息

批准号：
6555461
负责人：
CURTIS L MOSHER
金额：
$ 24.94万
依托单位：
BIOFORCE NANOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-07-01 至 2003-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6555461
关键词：
Bacillus anthracis antibody atomic force microscopy bacterial virus biomedical equipment development biosensor device biotechnology bioterrorism /chemical warfare microarray technology monitoring device nanotechnology

项目摘要

DESCRIPTION (provided by applicant): Rapid detection of biological warfare agents is one of the first lines of defense against bioterrorism. The development of multiplexed sensors capable of detecting any known biological warfare agent will be essential to rapidly implement measures to protect the population and military personnel from biological attack. The goal of this proposal is to develop an ultraminiaturized, sensitive biological warfare agent detection system. BioForce's NanoArray chip technology is based on affinity capture of pathogens using solid phase antibody arrays and Atomic Force Microscopy (AFM). Antibodies to bacteria or viral pathogens are deposited in an addressable array on a solid surface of a chip. Binding of bacterial spores or viral particles to antibodies in defined spatial domains on the chip are detected by direct observation, using AFM, of a topographic change at that address, and the pathogen identity thereby determined. The advantage of our technology is that it has the potential to directly detect bacterial spores, such as anthrax, without any sample preparation and determine the number of spores in a sample, rapidly, accurately and with high specificity. We have already provided proof of principle that our technology can detect viral pathogens. We propose to adapt this technology to detect biological warfare agents. In these phase I studies, we will develop a NanoArray composed of antibodies against surface antigens that can detect simulants of biological warfare agents such as B. globigii and MS2 bacteriophage. Chemistries will be developed to attach these antisera to chip surfaces using similar procedures as we have employed to attach antibodies on chip surfaces to capture viral particles. We will use AFM to detect simulant binding to antibodies on the NanoArray. The sensitivity, reaction kinetics and specificity for AFM detection will be determined for each simulant alone, in combination or in the presence of other bacteria or viruses. The assay will be optimized for quantitation of pathogens. If these studies are successful, then in future studies (Phase II SBIR), we will develop a NanoArray/AFM system to detect all known biological warfare agents which can serve as a general sensor to protect the public and military personal from bioterrorism.

描述（由申请人提供）：生物战剂的快速检测是防范生物恐怖主义的第一道防线之一。开发能够探测任何已知生物战剂的多路传感器对于迅速采取措施保护人民和军事人员免遭生物攻击至关重要。这项建议的目标是开发一种超小型、灵敏的生物战剂探测系统。BioForce的NanoArray芯片技术基于使用固相抗体阵列和原子力显微镜（AFM）对病原体的亲和捕获。针对细菌或病毒病原体的抗体以可寻址阵列的形式沉积在芯片的固体表面上。通过使用AFM直接观察该地址处的地形变化来检测细菌孢子或病毒颗粒与芯片上限定空间域中的抗体的结合，并由此确定病原体身份。我们的技术的优势在于，它有可能直接检测细菌孢子，如炭疽，无需任何样品制备，并快速，准确和高特异性地确定样品中的孢子数量。我们已经提供了原理证明，我们的技术可以检测病毒病原体。我们建议采用这种技术来探测生物战剂。在这些I期研究中，我们将开发由针对表面抗原的抗体组成的NanoArray，该抗体可以检测生物战剂的模拟物，例如B。globibiacin和MS 2噬菌体。将开发化学方法，以使用与我们将抗体附着在芯片表面以捕获病毒颗粒类似的程序将这些抗血清附着在芯片表面。我们将使用AFM检测模拟物与NanoArray上抗体的结合。AFM检测的灵敏度、反应动力学和特异性将针对单独、组合或存在其他细菌或病毒的每种模拟物进行测定。将优化检测试剂盒以定量病原体。如果这些研究是成功的，那么在未来的研究（第二阶段SBIR），我们将开发一个纳米阵列/原子力显微镜系统，以检测所有已知的生物战剂，可以作为一个通用的传感器，以保护公众和军事人员免受生物恐怖主义。