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AFM Sensors to Detect Biological Warfare Agents

用于检测生物战剂的 AFM 传感器

基本信息

批准号：
6555461
负责人：
CURTIS L MOSHER
金额：
$ 24.94万
依托单位：
BIOFORCE NANOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-07-01 至 2003-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6555461
关键词：
Bacillus anthracis antibody atomic force microscopy bacterial virus biomedical equipment development biosensor device biotechnology bioterrorism /chemical warfare microarray technology monitoring device nanotechnology

项目摘要

DESCRIPTION (provided by applicant): Rapid detection of biological warfare agents is one of the first lines of defense against bioterrorism. The development of multiplexed sensors capable of detecting any known biological warfare agent will be essential to rapidly implement measures to protect the population and military personnel from biological attack. The goal of this proposal is to develop an ultraminiaturized, sensitive biological warfare agent detection system. BioForce's NanoArray chip technology is based on affinity capture of pathogens using solid phase antibody arrays and Atomic Force Microscopy (AFM). Antibodies to bacteria or viral pathogens are deposited in an addressable array on a solid surface of a chip. Binding of bacterial spores or viral particles to antibodies in defined spatial domains on the chip are detected by direct observation, using AFM, of a topographic change at that address, and the pathogen identity thereby determined. The advantage of our technology is that it has the potential to directly detect bacterial spores, such as anthrax, without any sample preparation and determine the number of spores in a sample, rapidly, accurately and with high specificity. We have already provided proof of principle that our technology can detect viral pathogens. We propose to adapt this technology to detect biological warfare agents. In these phase I studies, we will develop a NanoArray composed of antibodies against surface antigens that can detect simulants of biological warfare agents such as B. globigii and MS2 bacteriophage. Chemistries will be developed to attach these antisera to chip surfaces using similar procedures as we have employed to attach antibodies on chip surfaces to capture viral particles. We will use AFM to detect simulant binding to antibodies on the NanoArray. The sensitivity, reaction kinetics and specificity for AFM detection will be determined for each simulant alone, in combination or in the presence of other bacteria or viruses. The assay will be optimized for quantitation of pathogens. If these studies are successful, then in future studies (Phase II SBIR), we will develop a NanoArray/AFM system to detect all known biological warfare agents which can serve as a general sensor to protect the public and military personal from bioterrorism.

描述(申请人提供)：快速检测生物战剂是对抗生物恐怖主义的第一道防线之一。开发能够检测任何已知生物战剂的多路传感器对于迅速实施保护人民和军事人员免受生物攻击的措施至关重要。这项提案的目标是开发一种超小型、灵敏的生物战剂检测系统。Bioforce的纳米阵列芯片技术是基于使用固相抗体阵列和原子力显微镜(AFM)对病原体进行亲和捕获的。针对细菌或病毒病原体的抗体以可寻址的阵列形式沉积在芯片的固体表面上。细菌孢子或病毒颗粒与芯片上限定空间域中的抗体的结合是通过使用AFM直接观察该地址的地形变化来检测的，并由此确定了病原体身份。我们的技术的优势是，它有可能直接检测细菌孢子，如炭疽菌，而不需要任何样品准备，并快速、准确和高特异性地确定样本中的孢子数量。我们已经提供了我们的技术可以检测病毒病原体的原则证据。我们建议将这项技术应用于检测生物战剂。在这些第一阶段的研究中，我们将开发一种由针对表面抗原的抗体组成的纳米阵列，可以检测生物战剂的模拟物，如球形芽孢杆菌和MS2噬菌体。将开发出将这些抗血清连接到芯片表面的化学方法，使用与我们将抗体连接到芯片表面以捕获病毒颗粒的类似程序。我们将使用AFM来检测与纳米阵列上的抗体的模拟结合。AFM检测的灵敏度、反应动力学和特异度将针对每种模拟物单独、结合或在其他细菌或病毒存在时确定。该检测方法将针对病原体的定量进行优化。如果这些研究成功，那么在未来的研究中(第二阶段SBIR)，我们将开发一个纳米阵列/原子力显微镜系统来检测所有已知的生物战剂，该系统可以作为通用传感器，保护公众和军事人员免受生物恐怖主义的伤害。