Core--Molecular and protein

分子和蛋白质核心实验室将为PPG内项目所需的分子试剂和抗体的开发提供集中设施。因为整个应用程序中的各个项目将需要类似的分子和抗体试剂（例如，标记的蛋白质过表达克隆、纯化的蛋白质、调节蛋白的抗体等），并且因为分子核心实验室掌握了构建这些试剂的所有专业知识和基础载体，所以谨慎的做法是将分子和抗体试剂集中到一个中心位置。该策略应在项目本身内提供人员时间的有效利用，使研究者摆脱载体构建和验证以及初始抗体开发和表征的负担。核心将提供项目分子方面所需的所有设备和材料、技术援助、指导和咨询。实验室以编目和有序的方式保存着所有质粒和载体的库存。此外，该实验室还拥有可用于北方分析以及RT-PCR反应的人类RNA储备。人类RNA储备是与新细胞培养核心实验室主任Scott Randell博士合作制备的。

The Molecular and Protein Core Laboratory will provide a centralized facility for development of molecular reagents and antibodies as required for the Projects within the PPG. Because the individual Projects within the overall application will require similar molecular and antibody reagents (e.g., tagged proteins over-expression clones, purified proteins, antibodies to regulatory proteins, etc.), and because the Molecular Core Laboratory has in hand all of the expertise and base vectors for construction of these reagents, it is prudent to central the molecular and antibody reagents into one central location. This strategy should provide an efficient use of personnel time within the Projects themselves, ridding the investigators of the burden of vector construction and verification and of initial antibody development and characterization. The Core will provide access to all equipment and materials necessary for molecular aspects of the Projects, technical assistance, instruction and consultation. The laboratory maintains a stock of all plasmids and vectors in a catalogued and orderly fashion. In addition, the laboratory also has stocks of human RNA that can be used for northern analysis as well as RT-PCR reactions. The human RNA stocks are prepared in collaboration with Dr. Scott Randell, the director of the new Cell Culture Core Laboratory.