Core--Molecular and protein

分子和蛋白质核心实验室将为PPG内的项目所需的分子试剂和抗体的开发提供一个集中的设施。由于整个应用程序中的各个项目将需要类似的分子和抗体试剂(例如，标记蛋白过度表达克隆、纯化蛋白、针对调节蛋白的抗体等)，并且由于分子核心实验室拥有构建这些试剂的所有专业知识和基础载体，因此谨慎地将分子和抗体试剂集中在一个中央位置。这一战略应能有效地利用项目本身内的人员时间，使研究人员摆脱构建和验证载体以及初步抗体开发和表征的负担。该核心将提供获得项目分子方面所需的所有设备和材料、技术援助、指导和咨询。该实验室以编目和有序的方式保存所有质粒和媒介的库存。此外，该实验室还储存了可用于Northern分析和RT-PCR反应的人类RNA。人类RNA库是与新的细胞培养核心实验室主任斯科特·兰德尔博士合作准备的。

The Molecular and Protein Core Laboratory will provide a centralized facility for development of molecular reagents and antibodies as required for the Projects within the PPG. Because the individual Projects within the overall application will require similar molecular and antibody reagents (e.g., tagged proteins over-expression clones, purified proteins, antibodies to regulatory proteins, etc.), and because the Molecular Core Laboratory has in hand all of the expertise and base vectors for construction of these reagents, it is prudent to central the molecular and antibody reagents into one central location. This strategy should provide an efficient use of personnel time within the Projects themselves, ridding the investigators of the burden of vector construction and verification and of initial antibody development and characterization. The Core will provide access to all equipment and materials necessary for molecular aspects of the Projects, technical assistance, instruction and consultation. The laboratory maintains a stock of all plasmids and vectors in a catalogued and orderly fashion. In addition, the laboratory also has stocks of human RNA that can be used for northern analysis as well as RT-PCR reactions. The human RNA stocks are prepared in collaboration with Dr. Scott Randell, the director of the new Cell Culture Core Laboratory.