CORE--BIOCHEMISTRY

产品说明：（改编自申请人的叙述）生化核心实验室以比在商业或医院实验室中分析样品更有效和更具成本效益的方式进行常规生化分析。 集中的生化中心设施还确保了检测结果的一致性，为每个检测保留完整的集中记录和明确的质量控制指南。 核心将（1）建立并确保样本采集、贴标、储存和运输的统一方法;（2）为项目研究者提供可靠、可重复、统一和及时的尿液、血浆和组织样本分析;（3）保存硬拷贝记录，并将所有生化和激素测量结果输入电子表格;（4）监督和维护项目调查人员使用的核心共用设备，并培训项目调查人员实验室人员正确使用设备;（5）提供专业知识和设备，协助调查人员开发所需的新检测方法。 (End该核心设施将提供并确保统一的样品收集、可靠的分析、记录维护、共享设备维护以及新检测方法的开发。 在过去4年的项目中，从400多名患者和650只大鼠中收集和分析了26，568份样本。在18种不同的试验中分析了人类样品，而在11种不同的试验中分析了大鼠样品。 除了传统的生化分析（葡萄糖、脂质、肌酸酐、电解质等）之外，进行放射免疫测定和HPLC测量（儿茶酚胺、肾素、醛固酮、胰岛素、加压素）。 在资助期间还开发了新的测定方法，包括从人体样本中提取ANF的新方法，检查非裔美国人与法裔加拿大人血浆的冷冻活化速率以寻找血浆肾素活性的巨大差异，开发瘦素测定法，尿白蛋白的新EIA，以及将cGMP和cAMP的RIA转换为更高通量的微量滴定板EIA。 预计在未来的项目年中，将向实验室提交53 050个样本。这是该计划的重要组成部分。 这是非常良好的组织和良好的装备。 工作人员非常有能力，这将很好地服务于该计划。

DESCRIPTION: (Adapted from the applicant's narrative) The Biochemical Core laboratory performs routine biochemical analyses in a more efficient and cost effective manner than having the samples analyzed in commercial or hospital based labs. The centralized Biochemical Core facility also ensures uniformity of assay results, maintains complete, centralized records and well defined quality control guidelines for each assay. The core will (1) establish and ensure uniform methods for the collection, labeling, storage, and transport of samples; (2) provide reliable, reproducible, uniform and timely analyses of urine, plasma and tissue samples for the project investigators; (3) maintain hard copy records and to enter the results for all biochemical and hormone measurements on electronic spreadsheet; (4) oversee and maintain shared use equipment in the core that is utilized by project investigators and train personnel of project investigator's laboratories in the proper use of the equipment; and (5) provide expertise and equipment to assist investigators in developing new assays as needed. (End of Abstract) This core facility will provide and ensure uniform collection of samples, reliable analyses, maintenance of records, maintenance of shared equipment, and development of new assays. In the past 4 years of the project, 26,568 samples have been collected and analyzed from over 400 patients and 650 rats. Human samples have been analyzed in 18 different assays while rats samples have been analyzed in 11 different assays. In addition to the traditional biochemical assays (glucose, lipids, creatinine, electrolytes, etc.), radioimmunoassays and HPLC measurements were made (catecholamines, renin, aldosterone, insulin, vasopressin). New assays were also developed during the funding period including a new extraction method for ANF from human samples, examination of the rate of cryoactivation of African American vs. French Canadian plasma to look for the large differences seen in plasma renin activities, development of a leptin assay, a new EIA for urinary albumin, and conversion of RIA's for cGMP and cAMP to higher throughput microtiter plate EIA's. It is anticipated that in the coming project years 53,050 samples will be presented to the laboratory. This is an important component of this program. It is extremely well organized and well equipped. The personnel are very capable, and it will serve the program well.