CORE--BIOCHEMICAL AND MOLECULAR

该项目资助的生化核心实验室将提供一种 整合高度专业化、装备精良和专业的工作人员 能够进行各种放射免疫分析、生化的装置 以及项目调查人员所要求的分子程序。没有单程票。 仅由R01支持的调查员实验室将是 能够装备和配备这种分析资源或人员 世卫组织将使用放射化学方法分析样本中的P450和NOS活性 高效液相色谱法测定脑脊液中20-HETE和EETs的微透析效率 使用新的荧光高效液相色谱分析方法。该核心还将测量IP3、DAG、 脑和脑血管中的小胶质和雌激素产物。 此外，我们还将用免疫印迹和免疫印迹法检测P4504和NOS蛋白 使用竞争性RCPR测量分子测量。访问 中央设施中的分析专门知识和设备是必不可少的 每一个拟议的项目，并以大量的 建议的生物化学和分子测定 学习。

Biochemical core lab of this program project grant will provide a consolidated highly specialized well-equipped and professional staff unit capable of performing a variety of radio-immunoassay, biochemical and molecular procedures as required by project investigators. No single investigator laboratory supported with R01 support alone would be capable of equipping and staffing this analytical resource or personnel who will analyze samples for P450 and NOS activities using radiochemical HPLC assay measurements, 20-HETE and EETs in CSF microdialysis efficient using new fluorescent HPLC assays. This core will also measure IP3, DAG, microglial and estroglial products in the brain and cerebrovasculature. In addition, we will measure P4504 and NOS protein using immunoblot and measure molecular measurements with competitive RCPR. Access to the analytical expertise and equipment in the central facility is essential to each of the proposed projects and justified by the large number of biochemical and molecular determinations required for the proposed studies.