CORE--BIOCHEMICAL

描述（由申请人提供）： 该PPG的生化核心实验室成立于20多年前，作为一个资源机构，提供一个统一的、高度专业化的、设备齐全的和专业人员配备的单位，能够执行项目研究者要求的各种免疫测定和其他生化程序。在这段时间里，该实验室的利用率和资源能力有所增长，并从部门和机构获得了大量的资本投入，以购买核心中现有的设备，并维持该计划的开发和日常工作中使用的最先进技术。没有任何一个实验室能够单独获得R 01赠款的支持，并配备这一分析资源。该集中设施对于本项目每个拟议项目的成功至关重要，并且该设施的持续支持是合理的，因为拟议研究需要大量的生化和分析测定。集中化 生物化学中心的人员和设备使该实验室能够以比研究人员在其各自实验室内进行这些分析更有效和成本有效的方式进行常规生物化学分析。将该程序的所有分析工作整合到Core中也增加了样品加载量，使我们可以在升级和更换现有设备时自动化大多数分析。例如，我们所有的HPLC系统现在都配备了冷藏自动进样器，我们将大多数常规分光光度测定转换为在临床化学自动分析仪上运行，我们修改并重新编程以处理我们可以从大鼠和小鼠收集的样品体积。这大大减少了我们的样品周转时间，并通过减少技术人员的时间和允许工作在一天24小时/一周7天的时间表上进行来证明是非常具有成本效益的。

DESCRIPTION (provided by applicant): The Biochemical Core Laboratory of this PPG was established over 20 years ago as a resource facility to provide a consolidated, highly specialized, well equipped and professionally staffed unit capable of performing a variety of immunoassay and other biochemical procedures as required by Program Project Investigators. Over this time, the utilization and resource capacity of this lab has grown and has received substantial capital input from the department and institution to purchase the equipment now available in the core and to sustain the state-of-the-art technology used in the development and routine work of this program. No single laboratory supported with R01 grants alone would be capable of equipping and staffing this analytical resource. This centralized facility is critical to the success of each of the proposed projects of this program and the continued support of this facility is justified by the large number of biochemical and analytical determinations required for the proposed studies. Centralizing the personnel and equipment in the Biochemical Core allows this laboratory to perform routine biochemical analyses in a more efficient and cost effective manner than if the investigators had setup to perform these analyses within their individual laboratories. Consolidation of all of the analytical work of this program into the Core also increases sample loads to an extent that we can justify automating most assays as we upgrade and replace existing equipment. For example, all of our HPLC systems are now equipped with refrigerated autosamplers and we converted most of our routine spectrophotometric assays to run on an clinical chemistry autoanalyzer we modified and reprogrammed to work with the sample volumes we can collect from rats and mice. This has greatly reduced our sample turnaround times and has proved to be highly cost effective by reducing technician time and allowing the work to proceed on a 24hr a day/7 day a week schedule.