CORE--BIOCHEMICAL

核心--生化

• 批准号: 7367213
• 负责人: Richard J. Roman
• 金额: $ 30.25万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7367213
• 关键词: Arts; Biochemical; Biological Assay; Capital; Clinical Chemistry; Development; Equipment; Grant; High Pressure Liquid Chromatography; Human Resources; Immunoassay; Individual; Institution; Laboratories; Mus; Numbers; Procedures; Rattus; Research Personnel; Resources; Running; Sampling; Schedule; System; Technology; Time; Week; Work; cost effective; day; programs; success

DESCRIPTION (provided by applicant): The Biochemical Core Laboratory of this PPG was established over 20 years ago as a resource facility to provide a consolidated, highly specialized, well equipped and professionally staffed unit capable of performing a variety of immunoassay and other biochemical procedures as required by Program Project Investigators. Over this time, the utilization and resource capacity of this lab has grown and has received substantial capital input from the department and institution to purchase the equipment now available in the core and to sustain the state-of-the-art technology used in the development and routine work of this program. No single laboratory supported with R01 grants alone would be capable of equipping and staffing this analytical resource. This centralized facility is critical to the success of each of the proposed projects of this program and the continued support of this facility is justified by the large number of biochemical and analytical determinations required for the proposed studies. Centralizing the personnel and equipment in the Biochemical Core allows this laboratory to perform routine biochemical analyses in a more efficient and cost effective manner than if the investigators had setup to perform these analyses within their individual laboratories. Consolidation of all of the analytical work of this program into the Core also increases sample loads to an extent that we can justify automating most assays as we upgrade and replace existing equipment. For example, all of our HPLC systems are now equipped with refrigerated autosamplers and we converted most of our routine spectrophotometric assays to run on an clinical chemistry autoanalyzer we modified and reprogrammed to work with the sample volumes we can collect from rats and mice. This has greatly reduced our sample turnaround times and has proved to be highly cost effective by reducing technician time and allowing the work to proceed on a 24hr a day/7 day a week schedule.

描述（由申请人提供）： 该PPG的生化核心实验室成立于20年前，是一种资源机构，可提供一个合并，高度专业化，设备齐全且配备良好的配备人员，能够按照计划项目调查人员的要求执行各种免疫测定和其他生化程序。在此期间，该实验室的利用率和资源能力不断增长，并收到了部门和机构的大量资本投入，以购买现在可用的核心设备，并维持该计划开发和日常工作中使用的最先进技术。没有R01赠款支持的单一实验室将能够为该分析资源装备和人员配备。这种集中式设施对于该计划的每个拟议项目的成功至关重要，并且该设施的持续支持是由拟议的研究所需的大量生化和分析确定证明的。集中 与研究人员在其单个实验室内进行这些分析相比，生化核心中的人员和设备使该实验室可以以更有效和具有成本效益的方式进行常规的生化分析。将该程序的所有分析工作整合到核心方面也将样本负载增加到一定程度，即我们可以在升级和替换现有设备时证明自动化大多数测定法。例如，我们所有的HPLC系统现在都配备了冷藏自动采样器，我们将大多数常规分光光度测定法转换为在临床化学自动分析仪上运行，我们对我们进行了修改并进行了重编程以与可以从大鼠和小鼠那里收集的样品量一起使用。这大大减少了我们的样本周转时间，并通过减少技术人员的时间并允许工作每天24小时/每周7天进行。