CORE--BIOCHEMICAL

描述(由申请人提供)： PPG的生化核心实验室是在20多年前建立的，作为一个资源设施，提供一个整合的、高度专业化的、设备齐全的专业人员单位，能够执行计划项目调查人员所要求的各种免疫分析和其他生化程序。在这段时间里，这个实验室的利用率和资源能力得到了增长，并从部门和机构获得了大量资本投入，用于购买目前核心可用的设备，并保持在该项目的开发和日常工作中使用的最先进技术。没有一个单独的实验室能够单独得到R01赠款的支持来装备和配备这一分析资源。这种集中化的设施对该计划的每个拟议项目的成功都至关重要，而且拟议研究所需的大量生化和分析测定证明了继续支持该设施是合理的。集中管理 生化核心中的人员和设备使该实验室能够以更高效和更具成本效益的方式执行常规生化分析，而不是让研究人员在各自的实验室内进行这些分析。将该计划的所有分析工作整合到核心中也增加了样品负载，以至于我们可以证明在升级和更换现有设备时实现大多数分析的自动化是合理的。例如，我们所有的高效液相系统现在都配备了冷藏自动进样器，我们将大多数常规分光光度分析转换为在临床化学自动分析仪上运行，我们对其进行了修改和重新编程，以处理我们可以从大鼠和小鼠身上采集的样本量。这极大地缩短了我们的样本周转时间，并通过减少技术人员的时间并允许工作按每周7天、每天24小时的时间表进行，从而证明了极具成本效益。

DESCRIPTION (provided by applicant): The Biochemical Core Laboratory of this PPG was established over 20 years ago as a resource facility to provide a consolidated, highly specialized, well equipped and professionally staffed unit capable of performing a variety of immunoassay and other biochemical procedures as required by Program Project Investigators. Over this time, the utilization and resource capacity of this lab has grown and has received substantial capital input from the department and institution to purchase the equipment now available in the core and to sustain the state-of-the-art technology used in the development and routine work of this program. No single laboratory supported with R01 grants alone would be capable of equipping and staffing this analytical resource. This centralized facility is critical to the success of each of the proposed projects of this program and the continued support of this facility is justified by the large number of biochemical and analytical determinations required for the proposed studies. Centralizing the personnel and equipment in the Biochemical Core allows this laboratory to perform routine biochemical analyses in a more efficient and cost effective manner than if the investigators had setup to perform these analyses within their individual laboratories. Consolidation of all of the analytical work of this program into the Core also increases sample loads to an extent that we can justify automating most assays as we upgrade and replace existing equipment. For example, all of our HPLC systems are now equipped with refrigerated autosamplers and we converted most of our routine spectrophotometric assays to run on an clinical chemistry autoanalyzer we modified and reprogrammed to work with the sample volumes we can collect from rats and mice. This has greatly reduced our sample turnaround times and has proved to be highly cost effective by reducing technician time and allowing the work to proceed on a 24hr a day/7 day a week schedule.