CORE--CELL BIOLOGY

核心--细胞生物学

• 批准号: 6564921
• 负责人: Guy A. Zimmerman
• 金额: $ 24.55万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564921
• 关键词: adult respiratory distress syndrome; biomedical facility; cell line; human tissue; tissue /cell culture; tissue resource /registry

The Cell Biology Core will interact with and support each of the projects in the SCOR. The central aim will be to provide well-characterize primary human cells and cell lines for use as models and in cell biologic studies that are relevant to lung injury in acute respiratory distress syndrome (ARDS), as we have done in the current funding period. Also, as in the current funding period. the major cell types will include freshly isolated neutrophils, monocytes, and other leukocytes and primary cultures of human endothelial cells. In addition, we will also grow, characterize, and furnish to the projects a variety of cell lines for specialized studies, including a pulmonary endothelial cell line developed in the Core. We focus on human cell types, because of immediate relevance, but also will provide cultured cells or established cell lines from experimental animals when useful. Core personnel also advise SCOR investigators on cell biology protocols, help identify and develop new preparations, pilot and develop specialized techniques, assays, and models, and in some cases perform specialized cell biologic studies or assays with or for SCOR investigators. The core will foster and extend fundamental investigations in the SCOR that will lead to new knowledge of the biologic features of lung injury in ARDS.

细胞生物学核心将与每个项目互动并提供支持 在 SCOR 中。中心目标是提供具有良好特征的初级 用作模型和细胞生物学研究的人类细胞和细胞系 与急性呼吸窘迫综合征的肺损伤相关 （ARDS），正如我们在当前资助期间所做的那样。另外，如在 当前资助期限。主要细胞类型将包括新鲜分离的 中性粒细胞、单核细胞和其他白细胞以及人类原代培养物 内皮细胞。此外，我们还将培养、表征和 为项目提供各种用于专门研究的细胞系， 包括在 Core 中开发的肺内皮细胞系。我们 重点关注人类细胞类型，因为具有直接相关性，但也将 提供来自实验动物的培养细胞或已建立的细胞系 当有用的时候。核心人员还为 SCOR 研究人员提供细胞生物学方面的建议 协议，帮助识别和开发新的制剂，进行试点和开发 专门的技术、分析和模型，并且在某些情况下执行 使用 SCOR 或针对 SCOR 进行专门的细胞生物学研究或测定 调查人员。核心将促进和扩展基础调查 在 SCOR 中，这将带来关于生物特征的新知识 ARDS 中的肺损伤。