Design, production and evaluation of novel protein-based antimicrobial agents

新型蛋白质抗菌剂的设计、生产和评价

• 批准号: 2106413
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2106413
• 关键词: Design; production; evaluation; novel; protein

Both bacteriophage and bacteria are rich sources of enzymes that efficiently cleave peptidoglycan in a variety of places resulting in selective bacterial cell death. Using a combination of synthetic biology and bioconjugation techniques, we will design, produce and evaluate new hybrid structures that either: 1. have modified targeting abilities and are selective for new bacterial species including those that exhibit resistance to current antibiotics; 2. Are a generation of new biologics that can use alternative antibacterial modalities such as reactive oxygen species to kill bacterial selectively including those in biofilms. The new structures may find uses as tools in biochemical research, for decontaminating surfaces including those on medical devices and ultimately as antibacterial agents for use in animal or human health.

噬菌体和细菌都是酶的丰富来源，这些酶在各种位置有效地切割肽聚糖，导致选择性细菌细胞死亡。使用合成生物学和生物缀合技术的组合，我们将设计，生产和评估新的杂交结构，或者：1。具有改良的靶向能力，并且对新的细菌物种具有选择性，包括对当前抗生素表现出抗性的那些; 2.是新一代的生物制剂，可以使用替代抗菌模式，如活性氧，以杀死细菌选择性包括那些在生物膜。这种新结构可能会在生物化学研究中用作工具，用于净化表面，包括医疗设备上的表面，并最终用作动物或人类健康中的抗菌剂。