Singlet Oxygen Detector for Photodynamic Therapy

用于光动力治疗的单线态氧检测器

• 批准号: 6690667
• 负责人: STEVEN J DAVIS
• 金额: $ 39.28万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690667
• 关键词: biomedical equipment development; clinical research; human subject; laboratory rat; method development; neoplasm /cancer photoradiation therapy; neoplastic cell; oximetry; patient monitoring device; photomultiplier; photosensitizing agents; porphyrins; singlet oxygen

DESCRIPTION (provided by applicant): Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photosensitizers such as hematoporphyrin derivative (HPD) and other compounds to be selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in their metastable singlet delta state O2 (1delta) are believed to be the species that destroys cancerous cells during PDT. A real-time optical diagnostic for singiet oxygen in-vivo would be a valuable dosimeter during PDT treatment. Based on a successful Phase I program, Physical Sciences Inc. proposes to develop and test a real-time optical monitor for singlet oxygen. We will use diode laser technology and ultra-sensitive photon counting methods. Extensive in-vitro testing will be performed using several photosensitizers. Crucial in-vivo tests will also be performed on tumor models in laboratory rats. These tests will be performed with our collaborators at the Massachusetts General Hospital. Success will lead to a product for medical research in PDT. The successful demonstration of the diagnostic could lead to numerous applications. For example, one could study the photo-induced HPD process in much more detail. New porphyrin compounds could be screened for O2 (1delta) production efficiency. Finally a real time O2 (1delta) monitor during phototherapy could be an invaluable tool for improving the efficiency of cancer cell destruction.

描述（由申请人提供）：光动力疗法（PDT）是一种有前途的癌症治疗方法。PDT利用光敏剂如血卟啉衍生物（HPD）和其他化合物的亲和力选择性地保留在恶性肿瘤中。 当用光敏剂预处理的肿瘤用可见光照射时，发生光化学反应并破坏肿瘤细胞。处于亚稳态单重态δ状态的氧分子O2（1 δ）被认为是PDT过程中破坏癌细胞的物质。在PDT治疗过程中，实时光学诊断单氧在体内将是一个有价值的剂量计。基于一个成功的第一阶段计划，物理科学公司。建议开发和测试单线态氧的实时光学监测器。我们将使用二极管激光技术和超灵敏光子计数方法。将使用几种光敏剂进行广泛的体外试验。还将在实验室大鼠的肿瘤模型上进行关键的体内试验。这些测试将由我们的合作者在马萨诸塞州总医院进行。 成功将导致一种用于PDT医学研究的产品。诊断的成功演示可能会导致许多应用。例如，人们可以更详细地研究光诱导的HPD过程。新的卟啉化合物可以筛选O2（1 δ）的生产效率。最后，光疗过程中的真实的实时O2（1 δ）监测器可能是提高癌细胞破坏效率的宝贵工具。