Ultra Sensitive Monitor for Singlet Oxygen

光动力疗法（PDT）是一种很有前途的癌症治疗方法。PDT利用光敏剂如血卟啉衍生物（HPD）等化合物的亲和力，在恶性肿瘤中选择性保留。当用光敏剂预处理的肿瘤用可见光照射时，发生光化学反应，肿瘤细胞被破坏。在PDT过程中，处于亚稳单δ态O2 （1delta）的氧分子被认为是破坏癌细胞的物种。在PDT治疗过程中，单线态氧的实时光学诊断将是一种有价值的剂量计。物理科学公司提议开发和测试一种单线态氧光学监测器，该监测器的灵敏度比目前的检测技术提高了10/3。在这个第一阶段，我们建议用几种光敏剂在多种溶剂中进行体外试验，包括含有强单线态氧猝灭剂的水溶液。该诊断的成功演示可能会导致许多应用。例如，人们可以更详细地研究光诱导的HPD过程。新的卟啉化合物可以通过O2 （1delta）的生成效率进行筛选。最后，光疗期间的实时O2 （1delta）监测仪可能是提高癌细胞破坏效率的宝贵工具。建议的商业应用：有几种商业应用。首先，可以使用光纤技术制作O2 （1delta）生产的实时诊断工具。外科医生可以使用这个工具来测量和最大化治疗过程中产生的氧（1delta）。其次，可以开发一种工具来测试新的光活性化合物产生O2（1 δ）的能力。人们还可以将该工具用作大规模生产这些hpd相关化合物的质量控制设备。最后，它也可以为更好地理解PDT的机制和动力学提供有价值的研究工具。

Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photo-sensitizers such as hematoporphyrin derivative (HPD) and other compounds to be selectively retained in malignant tumors in malignant tumors. When tumors, pre-treated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in their metastable single delta state O2 (1delta) are believed to be the species that destroys cancerous cells during PDT. A real-time optical diagnostic for singlet oxygen in-vivo would be a valuable dosimeter during PDT treatment. Physical Sciences Inc. proposes to develop and test an optical monitor for singlet oxygen that provides 10/3 sensitivity improvement over current detection techniques. In this Phase I we propose in-vitro tests with several photosensitizers in numerous solvents, including aqueous solutions containing strong quenchers of singlet oxygen. The successful demonstration of the diagnostic could lead to numerous applications. For example, one could study the photo-induced HPD process in much more detail. New porphyrin compounds could be screened for O2 (1delta) production efficiency. Finally a real time O2 (1delta) monitor during phototherapy could be an invaluable tool for improving the efficiency of cancer cell destruction. PROPOSED COMMERCIAL APPLICATIONS: There are several commercial applications. First, a real-time diagnostic tool for O2 (1delta) production could be produced using fiber optic technology. A surgeon could use this tool to measure and maximize the O2 (1delta) produced during treatments. Secondly, a tool could be developed to test new photoactive compounds for their ability to produce O2(1delta). One could also use the tool as a quality control device in mass production of these HPD-related compounds. Finally, it could also be a valuable research tool for developing a better understanding of the mechanisms and kinetics in PDT.