Ultra Sensitive Monitor for Singlet Oxygen

超灵敏单线态氧监测仪

• 批准号: 6486238
• 负责人: STEVEN J DAVIS
• 金额: $ 9.99万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6486238
• 关键词: biomedical equipment development; method development; neoplasm /cancer photoradiation therapy; neoplastic cell; patient monitoring device; photomultiplier; photosensitizing agents; porphyrins; singlet oxygen

Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photo-sensitizers such as hematoporphyrin derivative (HPD) and other compounds to be selectively retained in malignant tumors in malignant tumors. When tumors, pre-treated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in their metastable single delta state O2 (1delta) are believed to be the species that destroys cancerous cells during PDT. A real-time optical diagnostic for singlet oxygen in-vivo would be a valuable dosimeter during PDT treatment. Physical Sciences Inc. proposes to develop and test an optical monitor for singlet oxygen that provides 10/3 sensitivity improvement over current detection techniques. In this Phase I we propose in-vitro tests with several photosensitizers in numerous solvents, including aqueous solutions containing strong quenchers of singlet oxygen. The successful demonstration of the diagnostic could lead to numerous applications. For example, one could study the photo-induced HPD process in much more detail. New porphyrin compounds could be screened for O2 (1delta) production efficiency. Finally a real time O2 (1delta) monitor during phototherapy could be an invaluable tool for improving the efficiency of cancer cell destruction. PROPOSED COMMERCIAL APPLICATIONS: There are several commercial applications. First, a real-time diagnostic tool for O2 (1delta) production could be produced using fiber optic technology. A surgeon could use this tool to measure and maximize the O2 (1delta) produced during treatments. Secondly, a tool could be developed to test new photoactive compounds for their ability to produce O2(1delta). One could also use the tool as a quality control device in mass production of these HPD-related compounds. Finally, it could also be a valuable research tool for developing a better understanding of the mechanisms and kinetics in PDT.

光动力学疗法（PDT）是一种很有前途的癌症治疗方法。PDT利用光敏剂如血卟啉衍生物（HPD）和其他化合物的亲和力选择性地保留在恶性肿瘤中。当用光敏剂预处理的肿瘤用可见光照射时，发生光化学反应并破坏肿瘤细胞。处于亚稳态单δ状态的氧分子O2（1 δ）被认为是PDT过程中破坏癌细胞的物质。单态氧在体内的实时光学诊断将是PDT治疗过程中有价值的剂量计。物理科学公司提出开发和测试一种用于单线态氧的光学监测器，其提供比当前检测技术高10/3的灵敏度。在第一阶段中，我们提出了在许多溶剂中使用几种光敏剂的体外试验，包括含有单线态氧强猝灭剂的水溶液。诊断的成功演示可能会导致许多应用。例如，人们可以更详细地研究光诱导的HPD过程。新的卟啉化合物可以筛选O2（1 δ）的生产效率。最后，光疗过程中的真实的实时O2（1 δ）监测器可能是提高癌细胞破坏效率的宝贵工具。建议的商业应用：有几个商业应用。首先，可以使用光纤技术生产用于O2（1delta）生产的实时诊断工具。外科医生可以使用该工具测量并最大化治疗期间产生的O2（1delta）。其次，可以开发一种工具来测试新的光活性化合物产生O2（1 δ）的能力。人们还可以使用该工具作为这些HPD相关化合物的大规模生产中的质量控制装置。最后，它也可能是一个有价值的研究工具，为发展一个更好地了解PDT的机制和动力学。