Measuring protein turnover in vivo in plant mitochondria and chloroplasts to identify protease targets

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This project plans to measure the rate at which proteins degrade inside plants by using stable isotopes of nitrogen and mass spectrometry analysis of isolated protein samples from different plant mutant lines. This will allow new insights into the in vivo role of specific proteases which are involved in regulating energy generating pathways in plant organelles and that are needed for light tolerance, adaptation to day length, growth and normal leaf development. The new information will aid us to develop approaches to alter quality control of the plant proteome as a tool for engineering energy processes in plants.