Measuring protein turnover in vivo in plant mitochondria and chloroplasts to identify protease targets

测量植物线粒体和叶绿体中的体内蛋白质周转以识别蛋白酶靶标

• 批准号: DP140101580
• 负责人: Dr Shaobai Huang
• 金额: $ 47.36万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-07-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140101580
• 关键词: Measuring; protein; turnover; vivo; plant

This project plans to measure the rate at which proteins degrade inside plants by using stable isotopes of nitrogen and mass spectrometry analysis of isolated protein samples from different plant mutant lines. This will allow new insights into the in vivo role of specific proteases which are involved in regulating energy generating pathways in plant organelles and that are needed for light tolerance, adaptation to day length, growth and normal leaf development. The new information will aid us to develop approaches to alter quality control of the plant proteome as a tool for engineering energy processes in plants.

该项目计划通过使用氮的稳定同位素和对来自不同植物突变株系的分离蛋白质样品进行质谱分析来测量蛋白质在植物内部降解的速率。这将使人们对特定蛋白酶的体内作用有新的认识，这些蛋白酶参与调节植物细胞器中的能量产生途径，并且是耐光性、适应日照长度、生长和正常叶片发育所需的。新的信息将帮助我们开发方法来改变植物蛋白质组的质量控制，作为植物中工程能量过程的工具。