Aneugen Screening in Optimized Yeast Strains
Aneugen Screening in Optimized Yeast Strains
批准号:
6655611
负责人:
Owen Hughes
金额:
$34.91万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-09-30 至 2004-07-31
中文摘要
描述(由申请人提供):虽然非整倍性负责
英文摘要
DESCRIPTION (provided by applicant): Although aneuploidy is responsible for
significant adverse human health conditions such as birth defects, mental
retardation, spontaneous abortions, and cancer, little is known about the
possible aneugenicity of the chemicals and drugs we are exposed to. In Phase I
work, a novel Saccharomyces cerevisiae-based chomosome nondisjunction assay was
developed for eventual use in high throughput screening. This nondisjunction
assay has a simple fluorometric readout and responds to known aneugens. Here,
the assay will be adapted to an economical high throughput format. In addition,
methods for increasing the predictive value of yeast-based tests will be
explored in two ways. First, methods for decreasing the pharmacokinetic
differences between yeast and mammalian cells including 1) increasing drug
uptake, 2) decreasing drug excretion, and 3) mimicking mammalian metabolism of
test compounds will be explored. Second, two different types of "first hit"
sensitizing mutations--centromere mutations and mitotic checkpoint mutations--
will be examined. Finally, the nondisjunction assay will be combined with a
DEL-like assay to allow simultaneous assessment of both aneugenicity and
carcinogenicity.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Advanced image processing software for membrane protein structure determination
-
批准号:7433959
-
项目类别:
-
资助金额:$20.7万
-
财政年份:2008
-
负责人:Owen Hughes
-
依托单位:
Ceriodaphnia DNA Microarrays
-
批准号:7407595
-
项目类别:
-
资助金额:$28.78万
-
财政年份:2002
-
负责人:Owen Hughes
-
依托单位:
Ceriodaphnia DNA Microarrays
-
批准号:7681641
-
项目类别:
-
资助金额:$26.53万
-
财政年份:2002
-
负责人:Owen Hughes
-
依托单位:
C elegans RNAi Arrays
-
批准号:6404269
-
项目类别:
-
资助金额:$12.59万
-
财政年份:2001
-
负责人:Owen Hughes
-
依托单位:
SITE-SPECIFIC RECOMBINATION TOOLS FOR C ELEGANS
-
批准号:6074306
-
项目类别:
-
资助金额:$11.35万
-
财政年份:2000
-
负责人:Owen Hughes
-
依托单位:
ANEUGEN SCREENING IN OPTIMIZED C. ELEGANS STRAINS
-
批准号:6147664
-
项目类别:
-
资助金额:$11.32万
-
财政年份:2000
-
负责人:Owen Hughes
-
依托单位:
海外基金