CORE--ANIMAL RESOURCE

核心--动物资源

• 批准号: 6663393
• 负责人: Bruce R Ksander
• 金额: $ 13.47万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663393
• 关键词: Chelonia; Primates; animal breeding; animal care; animal colony; biomedical facility; eye; ferrets; fish; guinea pigs; hamsters; veterinary science; vision

Abstract: Funds are requested by 30 vision scientists to support 4 research modules that facilitate and enhance interaction among the vision scientists in the various disciplines represented at the Schepens Eye Research Institute. The modules will extend endeavors of individual research programs by providing collaborative opportunities for projects in which investigators do not have expertise, funding, or technical capabilities. The modules are: Morphology, Animal Resource, Laboratory Computer Applications and Flow Cytometry. The Morphology Module will provide light and electron microscopy, confocal microscopy and image analysis capabilities and tissue preparation for histochemistry and in situ hybridization, thereby increasing of morphologic techniques to biochemists, pharmacologists, cell and molecular biologists, and immunologists who need correlative morphologic data for their research. The module houses and maintains shared equipment for morphologic work and is available to all members of the Core. The Animal Resource Module will provide expertise and assistance in animal surgical techniques and postoperative care, administration of anesthesia, drugs, and medications. In addition, it will also provide for daily animal husbandry that is required to maintain our high standards for animal housing, sanitation, and veterinary care. The Laboratory Computer Applications Module will provide support to individual and collaborative efforts by assisting in interfacing of microcomputers with lab equipment and by developing software for gathering, processing, and analyzing experimental data by microcomputer systems. The module will also provide assistance to projects involving image processing and analysis from several types of equipment sources. The module interacts actively with the Morphology and Flow Cytometry Modules supporting computer- based technology. The Flow Cytometry Module is a centralized service providing principle investigators and collaborators access to equipment and technical support for flow cytometry and cell sorting. Available to the investigators are the Coulter Epics XL flow cytometer and the Coulter ELITE EPICS ESP fluorescent cell sorting. Available to the investigators are the Coulter Epics XL flow cytometer and the Coulter ELITE EPICS ESP fluorescent cell sorter. The modules are staffed by personnel with specialized training in the respective fields and equipment. Each module is under the immediate supervision of an established, experienced investigator(s). The module heads constitute the Core Grant Committee, which is responsible to the Director of Research.

摘要：30位视觉科学家要求资金支持4个研究模块，这些研究模块有助于和增强Schepens Eye Research Institute代表的各个学科的视觉科学家之间的相互作用。这些模块将通过为研究人员没有专业知识，资金或技术能力的项目提供协作机会来扩大个人研究计划的努力。这些模块是：形态，动物资源，实验室计算机应用和流式细胞仪。形态模块将提供光和电子显微镜，共聚焦显微镜和图像分析能力以及组织化学的准备和组织准备，以进行组织化学和原位杂交，从而提高了需要生物化学家，药理学家，细胞和分子生物学家以及需要其治疗学家的生物化学家，细胞和分子生物学家的研究。该模块设有并维护用于形态学工作的共享设备，并可以向所有核心成员使用。动物资源模块将在动物外科手术技术和术后护理，麻醉，药物和药物治疗方面提供专业知识和帮助。此外，它还将提供日常畜牧业，这是维持我们的动物住房，卫生和兽医护理的高标准所需的。实验室计算机应用模块将通过协助将微型计算机与实验室设备进行连接以及开发用于收集，处理和分析微型计算机系统的实验数据的软件，从而为个人和协作工作提供支持。该模块还将为涉及几种设备来源的图像处理和分析的项目提供帮助。该模块与支持基于计算机的技术的形态和流式细胞仪模块积极相互作用。流式细胞仪模块是一项集中式服务，可提供原理研究人员和合作者访问设备以及用于流式细胞仪和细胞分类的技术支持。研究人员可用于COULTER EPICS XL流式细胞仪和Coulter Elite Epics Epts Esp荧光细胞分类。研究人员可用于COULTER EPICS XL流式细胞仪和Coulter Elite Epics Epts ESP荧光细胞分选仪。这些模块由在各个领域和设备中进行专门培训的人员配备。每个模块都受到既定，经验丰富的调查员的立即监督。模块负责人构成了核心赠款委员会，该委员会负责研究主管。