CORE--Information Technology

这项核心拨款将通过支持四个研究和服务模块，进一步推动明尼苏达大学17名视力研究者的研究目标。数字成像模块由两个部分组成，共聚焦显微镜设备和图像分析设备。该共聚焦设备将被Core Grant研究人员用于从组织学切片和活组织中获取图像。图像分析设施将用于处理、分析和制作共聚焦显微镜上获得的图像和研究实验室产生的图像的硬拷贝。数字成像经理将监督共聚焦和图像分析设备，并培训调查人员和工作人员使用这些设备。信息技术模块将为数字成像模块以及使用微型计算机的个别调查人员提供必要的技术支持。信息技术经理将维护核心补助金网络服务器，将向调查人员提供网络和微型计算机支持，并将创建调查人员数据的存档备份。组织学模块将提供一个设备齐全的组织学实验室和模块组织学家的服务，以核心补助金的调查员。该设施提供的程序包括眼组织的石蜡和冷冻切片，切片的苏木精和伊红染色，免疫组织化学，PCR，原位杂交，核酸酶保护试验，RNA提取，质粒设计和构建以及序列分析。机器车间模块将提供一个熟练的机械师的服务，以核心格兰特调查人员需要定制的设计和加工的设备和他们的研究组件。这些模块将通过一个专用计算机网络连接在一起，方便调查人员访问，并将提高设施的能力。每个模块将由nei资助的既定调查员指导。核心奖助金将由一个由奖助金PI和模块主任组成的咨询小组管理。通过提供研究和服务设施，核心基金将进一步推动明尼苏达大学视觉研究人员的研究工作。

This Core Grant will further the research aims of 17 vision investigators at the University of Minnesota by supporting four research and service modules. The Digital Imaging module, consists of two components, the Confocal Microscope facility and the Image Analysis facility. The Confocal facility will be used by Core Grant investigators to acquire images from histological sections and from live tissue. The Image Analysis facility will be used to process, analyze and produce hard copies of images acquired on the confocal microscope and images produced in investigator laboratories. The Digital Imaging manager will supervise the Confocal and Image Analysis facilities and train investigators and staff on use of the equipment The Information Technology module will provide essential technical support for the Digital Imaging module as well as for individual investigators using microcomputers. The Information Technology manager will maintain the Core Grant network server, will provide networking and microcomputer support to investigators, and will create archival backups of investigator data. The Histology module will provide a fully equipped histology laboratory and the services of the module histologist to Core Grant investigators. Procedures offered by the facility include paraffin and frozen sections of ocular tissue, hematoxylin and eosin staining of sections, immunohistochemistry, PCR, in situ hybridization, nuclease protection assays, RNA extraction, plasmid design and construction, and sequence analysis. The Machine Shop module will provide the services of a skilled machinist to Core Grant investigators who require custom design and machining of equipment and components of their research. The modules will be linked together by a dedicated computer network that will facilitate investigator access and will enhance the capabilities of the facilities. Each module will be directed by an established NEI-funded investigator. The Core Grant will be administered by an advisory group comprised of the grant PI and module directors. The Core Grant, by providing research and service facilities, will further the research efforts of vision researchers at the University of Minnesota.