CORE--Information Technology

这项核心拨款将通过支持四个研究和服务模块，促进明尼苏达大学17名视觉研究人员的研究目标。数字成像模块由两个组件组成，即共焦显微镜设备和图像分析设备。Core Grant公司的研究人员将使用共焦设备从组织切片和活组织中获取图像。图像分析设施将用于处理、分析和制作在共焦显微镜上获取的图像和在调查实验室产生的图像的硬拷贝。数字成像管理员将监督共焦和图像分析设施，并对调查人员和工作人员进行设备使用方面的培训。信息技术单元将为数字成像模块以及使用微型计算机的个人调查人员提供必要的技术支持。信息技术管理员将维护核心赠款网络服务器，向调查员提供联网和微型计算机支持，并为调查员数据创建档案备份。组织学单元将向Core Grant调查人员提供设备齐全的组织学实验室和单元组织学家的服务。该设备提供的程序包括眼组织的石蜡切片和冰冻切片，切片的苏木精和伊红染色，免疫组织化学，聚合酶链式反应，原位杂交，核酸酶保护分析，RNA提取，质粒设计和构建，以及序列分析。机械车间模块将为Core Grant调查人员提供熟练机械师的服务，这些调查人员需要对其研究中的设备和部件进行定制设计和加工。这些模块将通过一个专门的计算机网络连接在一起，该网络将便利调查员访问，并将加强设施的能力。每个模块将由NEI资助的一名既定调查员指导。核心赠款将由一个由赠款PI和单元主任组成的咨询小组管理。核心助学金通过提供研究和服务设施，将进一步推动明尼苏达大学视觉研究人员的研究工作。

This Core Grant will further the research aims of 17 vision investigators at the University of Minnesota by supporting four research and service modules. The Digital Imaging module, consists of two components, the Confocal Microscope facility and the Image Analysis facility. The Confocal facility will be used by Core Grant investigators to acquire images from histological sections and from live tissue. The Image Analysis facility will be used to process, analyze and produce hard copies of images acquired on the confocal microscope and images produced in investigator laboratories. The Digital Imaging manager will supervise the Confocal and Image Analysis facilities and train investigators and staff on use of the equipment The Information Technology module will provide essential technical support for the Digital Imaging module as well as for individual investigators using microcomputers. The Information Technology manager will maintain the Core Grant network server, will provide networking and microcomputer support to investigators, and will create archival backups of investigator data. The Histology module will provide a fully equipped histology laboratory and the services of the module histologist to Core Grant investigators. Procedures offered by the facility include paraffin and frozen sections of ocular tissue, hematoxylin and eosin staining of sections, immunohistochemistry, PCR, in situ hybridization, nuclease protection assays, RNA extraction, plasmid design and construction, and sequence analysis. The Machine Shop module will provide the services of a skilled machinist to Core Grant investigators who require custom design and machining of equipment and components of their research. The modules will be linked together by a dedicated computer network that will facilitate investigator access and will enhance the capabilities of the facilities. Each module will be directed by an established NEI-funded investigator. The Core Grant will be administered by an advisory group comprised of the grant PI and module directors. The Core Grant, by providing research and service facilities, will further the research efforts of vision researchers at the University of Minnesota.