BORRELIA BURGDORFERI GENE EXPRESSION IN TICK MIDGUT--ROL

描述（取自应用程序）： 该研究应用旨在研究基因表达模式的 螺旋体疏螺旋体在硬蜱媒介，并了解分子 发生在螺旋体上的变化，以促进疾病的传播。 为实现这一目标，将采用一种新的方法来研究差异基因 蜱适应性螺旋体的表达。将进行实验以：A） 鉴定在特定的进食时间点由螺旋体表达的基因; B） 分析这些基因表达的时间和空间模式， 努力专注于与生物学相关的分子， C）产生缺乏特异性抗原的突变疏螺旋体， 本申请中鉴定的感兴趣的基因，并测试 这些变异的疏螺旋体来传播疾病基因的蛋白质产物 被推断在疾病传播中有生理作用， 最终作为阻断传播的疫苗进行测试。的结果 研究将使人们能够更好地了解参与 莱姆病的传播，并允许新的干预策略 疾病。调查还将有利于制定一项 有用的分子工具/方法来分析基因表达模式的其他 病媒环境中的病媒传播病原体。

DESCRIPTION (Taken from the application): The research application aims to study the gene expression pattern of the spirochete Borrelia in the Ixodes tick vector and to comprehend the molecular changes that occur on the spirochetes to facilitate disease transmission. Towards this goal, a novel approach will be utilized to study differential gene expression of tick-adapted spirochetes. Experiments will be carried out to: A) identify genes expressed by the spirochetes at specific feeding time points; B) analyze the temporal and spatial pattern of expression of these genes in an effort to focus on molecules that would be biologically relevant to transmission of disease; and C) to generate mutant Borrelia lacking specific genes of interest identified in this application and test the potential of these mutant Borrelia to transmit disease. The protein products of the genes that are inferred to have a physiological role in disease transmission will ultimately be tested as transmission blocking vaccines. The results of this investigation will enable a better understanding of molecules involved in transmission of Lyme disease and permit novel strategies for the intervention of disease. The investigation will also be conducive to the development of a useful molecular tool/approach to analyze the gene expression patterns of other vector-borne pathogens in their vector environment.