Tick gene expression- in the context of Lyme disease

蜱基因表达——在莱姆病的背景下

• 批准号: 6877083
• 负责人: SUKANYA NARASIMHAN
• 金额: $ 23.89万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877083
• 关键词: Borrelia; Ixodes; Lyme disease; RNA interference; bacteria infection mechanism; bacterial proteins; complementary DNA; double stranded RNA; fluorescence microscopy; gene expression; gene induction /repression; host organism interaction; laboratory mouse; microarray technology; polymerase chain reaction; salivary glands; ticks; two dimensional gel electrophoresis

DESCRIPTION (provided by the applicant): This R21 proposal builds upon the observations made in the RO1 (A1032947-11) which indicates that the expression of TROSPA, a tick receptor for OspA, is up regulated upon infection by Borrelia burgdorferi (B. burgdorferi), the causative agent of Lyme disease. This presumably facilitates Borrelia colonization of the tick midgut. B. burgdorferi likely modulates the tick transcriptome to ensure its survival and transmission. This aspect of Lyme disease has not been investigated and could open novel venues to vector and disease control. The proposed project aims to broaden the scope of the RO1 and shift the focus beyond TROSPA and OspA. Using cDNA arrays the global changes that occur in the tick salivary gland transcriptome during the infection and transmission of B. burgdorferi will be defined. The functional significance of these gene products in enabling B. burgdorferi invasion of tick salivary glands and its subsequent transmission will be evaluated by specifically silencing the selected tick genes in vivo, using the RNA interference technology. The results of this project will provide insights into tick-Borrelia interactions and enable identification of novel tick -based targets for blocking transmission of Lyme disease. This study will also serve to develop the infrastructure and genetic tools essential for making rapid progress in the field of tick functional genomics. This investigation may therefore serve to pave the way for elucidation of mechanisms underlying transmission of other tick-borne pathogens.

描述（由申请人提供）：该R21提案建立在RO1 （A1032947-11）中的观察基础上，该观察表明，在莱姆病病原体伯氏疏螺旋体（B. burgdorferi）感染后，OspA的蜱受体TROSPA的表达上调。这可能促进了伯氏疏螺旋体在蜱虫中肠的定植。伯氏疏螺旋体可能调节蜱的转录组以确保其存活和传播。