Population dynamics of sporogony in Thailand

泰国孢子生殖种群动态

• 批准号: 6654878
• 负责人: Jefferson Archer Vaughan
• 金额: $ 28.26万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6654878
• 关键词: Aedes; Plasmodium falciparum; animal population study; arthropod borne communicable disease; clinical research; communicable disease transmission; disease vectors; epizootiology; field study; genetic polymorphism; genotype; human subject; life cycle; malaria; parasitism; southeast Asia; species difference

DESCRIPTION (provided by the applicant): Malaria remains one of the most important infectious diseases in the world. Transmission of malaria is contingent on the sporogonic development of Plasmodium parasites within mosquitoes. Sporogony is a complex process involving several morphologically distinct life stages and can be described in terms of parasite population dynamics. Most of our knowledge about sporogony comes from laboratory model systems. There are very few studies that have attempted to examine in a rigorous, quantitative fashion the process of sporogony as it occurs in nature. The major hypotheses of this proposal are that 1) parasite sporogony is more efficient in the field than in the laboratory and 2) there are key life stage transitions that determine the probability of sporogonic success within the vector (i.e., developmental bottlenecks). The magnitude and anatomical site of these bottlenecks within the mosquito may differ for different parasites developing in different mosquito species. The objective of this proposal is to use a population dynamics approach to identify the key life stage transitions that most strongly influence the success of Plasmodium falciparum and P. vivax sporogony within indigenous vector populations. The experimental approach will involve controlled mosquito infections with naturally occurring Plasmodium falciparum and P. vivax parasites, using local anopheline species. The parasite populations developing within infected mosquitoes will be sampled throughout sporogonic development and the densities of key parasite life stages (i.e., gametocytes, ookinetes, oocysts and sporozoites) will be quantified. The relative efficiencies of life stage transitions (changes in abundance) and the degree of heterogeneity in mosquito susceptibility to infection (frequency distributions) will be compared among mosquito infections. The proposed studies will be conducted in a small village in western Thailand. Studies are in collaboration with existing U.S. military field research efforts and the Thai Ministry of Public Health in an effort to understand how the population dynamics of sporogony correlate to the epidemiology of local malaria transmission. This project represents the first long-term examination of the natural population dynamics of malaria parasites within their natural vectors and will lead to a better understanding of how plasmodial parasites survive and are maintained within mosquitoes in the field.

描述（由申请人提供）：疟疾仍然是最多的 世界上重​​要的传染病。疟疾的传播是 疟原虫寄生虫的孢子形发展 蚊子。孢子形是一个复杂的过程，涉及多个形态学 不同的生活阶段，可以用寄生虫种群来描述 动力学。我们关于孢子虫的大多数知识来自实验室模型 系统。很少有研究试图检查 严格，定量的方式是自然界中发生的孢子虫的过程。 该提议的主要假设是1）寄生虫孢子虫更多 在现场高于实验室和实验室的效率2）有关键的生命阶段 确定孢子形成功概率的过渡 向量（即发育瓶颈）。大小和解剖位点 蚊子内的这些瓶颈可能在不同的寄生虫中有所不同 在不同的蚊子中发育。该提议的目的是 使用人群动态方法来识别关键生命阶段过渡 最强烈影响恶性疟原虫和维瓦克斯疟原虫的成功 土著载体种群中的孢子形成。实验方法将 涉及自然存在的疟原虫受控的蚊子感染 恶性疟原虫和维瓦克斯寄生虫，使用局部障碍物种。寄生虫 在被感染的蚊子中发展的种群将在整个过程中进行采样 孢子形的发展和关键寄生虫生命阶段的密度（即 将量化配子细胞，Ookinetes，卵囊和孢子菌）。这 生命阶段过渡的相对效率（丰度变化）和 蚊子对感染敏感性的异质性程度（频率 将在蚊子感染之间比较分布）。提出的研究 将在泰国西部的一个小村庄进行。研究正在进行中 与现有的美国军事现场研究工作和泰国合作 公共卫生部是为了了解人口如何 孢子虫的动态与局部疟疾的流行病学相关 传播。该项目代表了对 自然媒介中疟疾的自然人口动态 并将更好地理解质寄生虫的生存和 维持在田间的蚊子中。

项目成果

Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand.

• DOI: 10.1186/1475-2875-5-121
• 发表时间: 2006-12-14
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Coleman RE;Sattabongkot J;Promstaporm S;Maneechai N;Tippayachai B;Kengluecha A;Rachapaew N;Zollner G;Miller RS;Vaughan JA;Thimasarn K;Khuntirat B
• 通讯作者: Khuntirat B

Rodent Plasmodium: population dynamics of early sporogony within Anopheles stephensi mosquitoes.

啮齿类疟原虫：史氏按蚊早期孢子生殖的种群动态。

• DOI: 10.1645/ge-1407.1
• 发表时间: 2008
• 期刊: The Journal of parasitology
• 作者: Poudel,ShreekantaS;Newman,RobertA;Vaughan,JeffersonA
• 通讯作者: Vaughan,JeffersonA

Rhabdias kongmongthaensis sp. n. (Nematoda: Rhabdiasidae) from Polypedates leucomystax (Amphibia: Anura: Rhacophoridae) in Thailand.

Rhabdias kongmongthaensis sp.

• DOI: 10.14411/fp.2005.046
• 发表时间: 2005
• 期刊: Folia parasitologica
• 影响因子: 1.6
• 作者: Kuzmin,Yuriy;Tkach,VasylV;Vaughan,JeffersonA
• 通讯作者: Vaughan,JeffersonA

Plasmodium falciparum: Genetic diversity and complexity of infections in an isolated village in western Thailand

恶性疟原虫：泰国西部一个偏僻村庄的遗传多样性和感染复杂性

• DOI: 10.1016/j.parint.2013.09.011
• 发表时间: 2013
• 期刊: Parasitol Int
• 影响因子: 1.9
• 作者: K. Tanabe;G. Zollner;J. A. Vaughan;J. Sattabongkot;B. Khuntirat;H. Honma;T. Mita;T. Tsuboi and R. Coleman
• 通讯作者: T. Tsuboi and R. Coleman

A new species of blood fluke (Digenea: Spirorchiidae) from the Malayan box turtle, Cuora amboinensis (Cryptodira: Geomydidae) in Thailand.

来自泰国马来闭壳龟 Cuora amboinensis（Cryptodira：Geomydidae）的血吸虫新种（Digenea：Spirorchiidae）。

• DOI: 10.1645/ge-1858.1
• 发表时间: 2009
• 期刊: The Journal of parasitology
• 作者: Tkach,VasylV;Snyder,ScottD;Vaughan,JeffersonA
• 通讯作者: Vaughan,JeffersonA