CORE--CELL CULTURE

核心--细胞培养

• 批准号: 6564816
• 负责人: SANDRA R BATES
• 金额: $ 22.37万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-10 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564816
• 关键词: alveolar macrophages; biomedical facility; laboratory rat; pulmonary surfactants; respiratory epithelium; respiratory function; tissue /cell culture

The cell culture Core laboratory will serve as tissue culture resource for this Program Project. It will isolate primary cells for culture and maintain various cell lines. Type II cells isolated from neonatal and adult rats will be the principal cell type prepared. In addition, Core D will develop and characterize methods for the isolation and culture of pneumocytes which retain or recover important features of alveolar type II cells. especially those related to surfactant metabolism. The Core is located in the main IFEM laboratory and contains all equipment necessary for cell maintenance and propagation including incubators, laminar flow hoods,, inverted microscopes, a sterile room, and an autoclave. The personnel will include the Director, Dr. Sandra Bates, who has extensive experience in cell-culture, Jain-Qin Tao, the tissue culture technician, and Daisy Serrano, who will aid in cell culture and prepare media and glassware.

细胞培养核心实验室将作为本计划项目的组织培养资源。它将分离原代细胞进行培养，并保持各种细胞系。从新生大鼠和成年大鼠分离的II型细胞将是主要的细胞类型。此外，Core D将开发和鉴定分离和培养保留或恢复肺泡II型细胞重要特征的肺泡细胞的方法。尤其是与表面活性物质代谢有关的。核心位于IFEM主实验室，包含细胞维护和繁殖所需的所有设备，包括培养箱、层流罩、倒置显微镜、灭菌室和高压灭菌器。这些人员将包括主任桑德拉·贝茨博士，她在细胞培养方面拥有丰富的经验，组织培养技术员贾恩-秦涛，以及黛西·塞拉诺，她将协助细胞培养，准备媒体和玻璃器皿。