CORE--CELL CULTURE

核心--细胞培养

• 批准号: 6564816
• 负责人: SANDRA R BATES
• 金额: $ 22.37万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-10 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564816
• 关键词: alveolar macrophages; biomedical facility; laboratory rat; pulmonary surfactants; respiratory epithelium; respiratory function; tissue /cell culture

The cell culture Core laboratory will serve as tissue culture resource for this Program Project. It will isolate primary cells for culture and maintain various cell lines. Type II cells isolated from neonatal and adult rats will be the principal cell type prepared. In addition, Core D will develop and characterize methods for the isolation and culture of pneumocytes which retain or recover important features of alveolar type II cells. especially those related to surfactant metabolism. The Core is located in the main IFEM laboratory and contains all equipment necessary for cell maintenance and propagation including incubators, laminar flow hoods,, inverted microscopes, a sterile room, and an autoclave. The personnel will include the Director, Dr. Sandra Bates, who has extensive experience in cell-culture, Jain-Qin Tao, the tissue culture technician, and Daisy Serrano, who will aid in cell culture and prepare media and glassware.

细胞培养核心实验室将作为该计划项目的组织培养资源。它将分离原代细胞以进行培养并维持各种细胞系。从新生儿和成年大鼠分离的II型细胞将是准备的主要细胞类型。 此外，核心D将开发并表征用于保留或恢复肺泡II型细胞重要特征的肺细胞分离和培养的方法。尤其是与表面活性剂代谢有关的。核心位于主IFEM实验室中，并包含细胞维护和繁殖所需的所有设备，包括孵化器，层流罩，倒置显微镜，无菌室和高压灭菌器。这些人员将包括主任桑德拉·贝茨（Sandra Bates）博士，他在细胞文化，Jain-Qin Tao，组织培养技术员和Daisy Serrano方面拥有丰富的经验，他们将帮助细胞培养并准备媒体和玻璃器皿。