CORE--Cell Culture and Animal Husbandry

核心--细胞培养与畜牧业

• 批准号: 6968190
• 负责人: SANDRA R BATES
• 金额: $ 11.62万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6968190
• 关键词: MCF7 cell; alveolar macrophages; animal colony; biomedical facility; cell line; cell sorting; cell type; gene targeting; genetically modified animals; laboratory mouse; pulmonary artery; respiratory epithelium; tissue /cell culture; tissue resource /registry; vascular endothelium

The Cell Culture and Animal Husbandry laboratory will serve as a source for biologic materials. It will provide cell culture facilities and expertise as well as animal husbandry service. Specifically, we will isolate and maintain various cells types from the lungs of wild-type and gene-targeted mice including pulmonary microvascular endothelial cells, type II epithelial cells and alveolar macrophages; will culture umbilical vein and pulmonary artery endothelial cells, will culture the established breast cancer cell line MCF7 to be used in testing the efficacy of mutant and antioxidant enzymes ; and will carry various cell lines to be used for the testing of transcription factors and promoter mapping. In addition, we will provide support for the maintenance of the seven mutant mice colonies planned including documentation and coordination for the breeding of the gene-targeted mice. We will be located in the main IFEM laboratory which contains all equipment necessary for these functions including incubators, centrifuges, laminar flow hoods, inverted microscopes, sterile rooms, freezers, fume hoods, oxygen chambers for mice and an autoclave. The personnel will include the Director, Dr. Sandra Bates and Mrs. Kristine De Bolt, the technician who will perform both tissue culture technician and animal husbandry services.

细胞培养和畜牧实验室将作为生物材料的来源。它将提供细胞培养设施和专业知识以及畜牧业服务。 具体而言，我们将从野生型和靶向基因的小鼠的肺中分离并维护各种细胞类型，包括肺微血管内皮细胞，II型上皮细胞和肺泡巨噬细胞；将培养脐静脉和肺动脉内皮细胞，将培养已建立的乳腺癌细胞系MCF7，用于测试突变体和抗氧化剂酶的功效。并将携带各种细胞系，用于测试转录因子和启动子映射。此外，我们将为维持计划的七个突变小鼠菌落的维护提供支持，包括文档和协调基因靶向小鼠的繁殖。我们将位于主要IFEM实验室中，其中包含这些功能所需的所有设备，包括孵化器， 离心机，层流罩，倒立显微镜，无菌房间，冷冻机，通风罩，小鼠的氧气室和一只高压灭菌器。这些人员将包括董事，桑德拉·贝茨（Sandra Bates）博士和克里斯汀·德·博尔特（Kristine de Bolt）夫人，他们将同时执行组织文化技术人员和畜牧业服务。