CORE--Cell Culture and Animal Husbandry

核心--细胞培养与畜牧业

• 批准号: 6968190
• 负责人: SANDRA R BATES
• 金额: $ 11.62万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6968190
• 关键词: MCF7 cell; alveolar macrophages; animal colony; biomedical facility; cell line; cell sorting; cell type; gene targeting; genetically modified animals; laboratory mouse; pulmonary artery; respiratory epithelium; tissue /cell culture; tissue resource /registry; vascular endothelium

The Cell Culture and Animal Husbandry laboratory will serve as a source for biologic materials. It will provide cell culture facilities and expertise as well as animal husbandry service. Specifically, we will isolate and maintain various cells types from the lungs of wild-type and gene-targeted mice including pulmonary microvascular endothelial cells, type II epithelial cells and alveolar macrophages; will culture umbilical vein and pulmonary artery endothelial cells, will culture the established breast cancer cell line MCF7 to be used in testing the efficacy of mutant and antioxidant enzymes ; and will carry various cell lines to be used for the testing of transcription factors and promoter mapping. In addition, we will provide support for the maintenance of the seven mutant mice colonies planned including documentation and coordination for the breeding of the gene-targeted mice. We will be located in the main IFEM laboratory which contains all equipment necessary for these functions including incubators, centrifuges, laminar flow hoods, inverted microscopes, sterile rooms, freezers, fume hoods, oxygen chambers for mice and an autoclave. The personnel will include the Director, Dr. Sandra Bates and Mrs. Kristine De Bolt, the technician who will perform both tissue culture technician and animal husbandry services.

细胞培养和动物饲养实验室将作为生物材料的来源。它将提供细胞培养设施和专业知识以及畜牧业服务。 具体而言，我们将从野生型和基因靶向小鼠的肺中分离和维持各种类型的细胞，包括肺微血管内皮细胞、II型上皮细胞和肺泡巨噬细胞;将培养脐静脉和肺动脉内皮细胞，将培养已建立的乳腺癌细胞系MCF 7以用于测试突变体和抗氧化酶的功效;并将携带用于测试转录因子和启动子作图的各种细胞系。此外，我们将为计划中的7个突变小鼠群的维护提供支持，包括基因靶向小鼠育种的文件编制和协调。我们将位于IFEM的主要实验室，其中包含所有必要的设备，包括孵化器， 离心机、层流罩、倒置显微镜、无菌室、冷冻机、通风柜、小鼠氧气室和高压灭菌器。工作人员将包括主任Sandra Bates博士和Kristine De Bolt夫人，技术人员将执行组织培养技术员和畜牧服务。