CORE--CELL CULTURE

细胞培养核心实验室将作为本计划项目的组织培养资源。它将分离用于培养的原代细胞并维持各种细胞系。从新生和成年大鼠中分离的II型细胞将是制备的主要细胞类型。 此外，核心D将开发和表征用于分离和培养保留或恢复肺泡II型细胞重要特征的肺细胞的方法。特别是与表面活性剂代谢相关的那些。核心位于IFEM主实验室，包含细胞维持和繁殖所需的所有设备，包括培养箱、层流罩、倒置显微镜、无菌室和高压灭菌器。工作人员将包括在细胞培养方面具有丰富经验的主任Sandra Bates博士、组织培养技术员Jain-Qin Tao和协助细胞培养并准备培养基和玻璃器皿的Daisy塞拉诺。

The cell culture Core laboratory will serve as tissue culture resource for this Program Project. It will isolate primary cells for culture and maintain various cell lines. Type II cells isolated from neonatal and adult rats will be the principal cell type prepared. In addition, Core D will develop and characterize methods for the isolation and culture of pneumocytes which retain or recover important features of alveolar type II cells. especially those related to surfactant metabolism. The Core is located in the main IFEM laboratory and contains all equipment necessary for cell maintenance and propagation including incubators, laminar flow hoods,, inverted microscopes, a sterile room, and an autoclave. The personnel will include the Director, Dr. Sandra Bates, who has extensive experience in cell-culture, Jain-Qin Tao, the tissue culture technician, and Daisy Serrano, who will aid in cell culture and prepare media and glassware.