SOLUBLE ADENYLYL CYCLASE ACTIVITY IN MALE GERM CELLS

雄性生殖细胞中可溶性腺苷酸环化酶活性

• 批准号: 6629134
• 负责人: LONNY R LEVIN
• 金额: $ 30.51万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2005-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6629134
• 关键词: acrosome; adenosine triphosphate; adenylate cyclase; bicarbonates; biological signal transduction; embryonic stem cell; enzyme activity; enzyme inhibitors; enzyme mechanism; enzyme structure; estrogens; fertility; gene targeting; genetically modified animals; immunoprecipitation; isozymes; laboratory mouse; protein purification; solubility; sperm; sperm capacitation; sperm motility; spermatogenesis; testis; yeast two hybrid system

DESCRIPTION: (Adapted from the applicant's abstract) cAMP, a nearly universal signaling molecule, is synthesized from ATP by a broad family of adenylyl cyclases (AC). In mammals, there are two types of AC. One form spans the membrane and is encoded by a gene family consisting of nine related transmembrane ACs (tmAC). These are regulated by G proteins and other second messengers. The P.I. has recently identified a second type of mammalian AC, soluble AC (sAC) that is insensitive to the known modulators of tmACs and does not possess membrane spanning domains. tmAC and sAC appear to mediate opposite effects on cellular proliferation and are differentially modulated by pharmacological agents. sAC activity is directly regulated by bicarbonate ions. Since sAC is preferentially expressed in male germ cells and is the only known bicarbonate stimulated adenylyl cyclase in mammals, the investigators propose that it is an excellent candidate to play roles in aspects of sperm motility, capacitiation and the acrosome reaction. The proposed studies will determine the regulatory and enzymological profile of sAC and use pharmacological and genetic approaches to test the role of sAC in reproductive function. Long term plans include developing sAC as a potential target for pharmacological therapeutics affecting fertility.

描述：（改编自申请人的摘要）cAMP，一个几乎普遍的