Acoustic Separation of Cancer-derived Extracellular Vesicles for Cancer Early Diagnostics

声学分离癌症来源的细胞外囊泡用于癌症早期诊断

• 批准号: 2117761
• 金额: --
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2117761
• 关键词: Acoustic; Separation; Cancer; derived; Extracellular

EPSRC Portfolio Research Areas: The project aligns with RF and microwave devices (Grow), Sensors and Instrumentation (Maintain), Microsystems (Maintain), and Clinical Technologies (Maintain). Description:A cancer becomes life-threatening when the primary tumour spreads from the place where it first started to another place in the body. The spreading process is called metastasis, a tumour formed by spreading cancer cells is called a metastatic tumour. Metastasis is the principal cause of death in patients diagnosed with invasive cancer. At present, there is no curative treatment available for patients with metastatic cancer. This clearly demonstrates the urgent need for increased knowledge about the cellular communication mechanisms between tumour cells and normal cells resulting in pathological metabolism in the metastatic site. Extracellular vesicles (EVs) are a distinct population of membranous vesicles of endocytic origin. They range from 30 to 150 nm in diameter and are released by cells upon fusion of intracellular multivesicular bodies with the plasma membrane. Acoustic waves have been applied in highly sensitive separation of bio-particles such as cells. By increasing the frequency of the acoustic waves, they are capable of separating cancer-derived EVs subpopulation from whole blood. The proposed project, built on the supervisors' available techniques, will fabricate a high frequency acoustic transducer integrating acoustic and microfluidic techniques to identify and separate cancer-derived EV subpopulation from other EVs. The method will be evaluated against conventional EV separation by ultracentrifugation to investigate its sensitivity and specificity. Cancer patient blood will be sourced to test the transducer in a preliminary clinical study. The transducer will be an integrative and sensitive alternative diagnostic technique that targets system-level circulating tumour biomarkers to allow the investigation of the fundamental roles of EVs in cancer progression and treatment response. The candidate will work in a multidisciplinary team including engineers, biologists and clinicians to investigate developing and implementing the novel transducer for new real-time diagnosis and classification of cancer by using EV as a cancer marker.

EPSRC 组合研究领域：该项目涉及射频和微波设备（增长）、传感器和仪器（维护）、微系统（维护）和临床技术（维护）。描述：当原发肿瘤从最初发生的地方扩散到体内的另一个地方时，癌症就会危及生命。扩散的过程称为转移，癌细胞扩散形成的肿瘤称为转移瘤。转移是诊断为浸润性癌症患者的主要原因。目前，对于转移性癌症患者尚无有效的治疗方法。这清楚地表明迫切需要增加对肿瘤细胞和正常细胞之间导致转移部位病理代谢的细胞通讯机制的了解。细胞外囊泡（EV）是一种独特的内吞来源的膜囊泡群体。它们的直径范围为 30 至 150 nm，在细胞内多泡体与质膜融合时由细胞释放。声波已应用于细胞等生物颗粒的高度灵敏分离。通过增加声波的频率，它们能够从全血中分离出癌症来源的 EV 亚群。拟议的项目建立在主管人员现有技术的基础上，将制造一种集成声学和微流体技术的高频声换能器，以识别癌症衍生的 EV 亚群并将其与其他 EV 分开。该方法将与传统的超速离心 EV 分离进行评估，以研究其敏感性和特异性。癌症患者的血液将用于在初步临床研究中测试换能器。该传感器将是一种综合且敏感的替代诊断技术，针对系统级循环肿瘤生物标志物，以研究 EV 在癌症进展和治疗反应中的基本作用。候选人将在一个包括工程师、生物学家和临床医生的多学科团队中工作，研究开发和实施新型传感器，通过使用 EV 作为癌症标记物来进行新的实时诊断和癌症分类。

项目成果

Gallium Nitride: A Versatile Compound Semiconductor as Novel Piezoelectric Film for Acoustic Tweezer in Manipulation of Cancer Cells

氮化镓：一种多功能化合物半导体，可用作声镊操作癌细胞的新型压电薄膜

• DOI: 10.1109/ted.2020.3002498
• 发表时间: 2020
• 期刊: IEEE Transactions on Electron Devices
• 影响因子: 3.1
• 作者: Sun C

Flexible Printed Circuit Board as Novel Electrodes for Acoustofluidic Devices

• DOI: 10.1109/ted.2020.3039760
• 发表时间: 2020-11
• 期刊: IEEE Transactions on Electron Devices
• 影响因子: 3.1
• 作者: Chao Sun;R. Mikhaylov;Yongqing Fu;Fangda Wu;Hanlin Wang;Xichen Yuan;Zhihua Xie;Dongfang Liang;Zhenlin Wu;Xin Yang

An Enhanced Tilted-Angle Acoustofluidic Chip for Cancer Cell Manipulation

用于癌细胞操作的增强型倾斜角声流控芯片

• DOI: 10.1109/led.2021.3062292
• 发表时间: 2021
• 期刊: IEEE Electron Device Letters
• 影响因子: 4.9
• 作者: Wu F