AMPA receptors plasticity regulated by stargazin

Stargazin 调节 AMPA 受体可塑性

• 批准号: 6650435
• 负责人: Susumu Tomita
• 金额: $ 4.81万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6650435
• 关键词: AMPA receptors; NMDA receptors; SDS polyacrylamide gel electrophoresis; biological signal transduction; chloride channels; electrophysiology; endocytosis; immunofluorescence technique; kinase inhibitor; laboratory mouse; laboratory rat; learning; long term potentiation; memory; neural plasticity; phosphorylation; postdoctoral investigator; tissue /cell culture; western blottings

DESCRIPTION (provided by applicant): The broad goal of this project is to help understand mechanisms for synaptic plasticity. One mechanism for long-term potentiation (LTP) or long-term depression (LTD) requires AMPA receptors recycling regulated by activation of protein phosphorylation downstream of the NMDA receptor. However, the specific cellular mechanisms that regulate AMPA receptors trafficking by synaptic activity remain largely unclear. To address this important problem, I have configured a biochemical strategy to characterize the machinery responsible for AMPA receptor trafficking by phosphorylation of stargazin, which is essential for functional AMPA receptors and is highly phosphorylated in brain. Due to the interdependence of the regulation of functional AMPA receptor by stargazin and the function of stargazin phosphorylation, this work has broad implications for mechanisms of learning and memory.

描述（由申请人提供）：该项目的广泛目标是帮助了解突触可塑性的机制。长期增强（LTP）或长期抑郁症（LTD）的一种机制，需要通过NMDA受体下游蛋白质磷酸化的激活调节的AMPA受体回收。但是，通过突触活动调节AMPA受体运输的特定细胞机制仍然在很大程度上不清楚。为了解决这个重要的问题，我配置了一种生化策略，以表征通过Stargazin磷酸化负责AMPA受体运输的机械，这对于功能性AMPA受体至关重要，并且在大脑中高度磷酸化。由于Stargazin对功能AMPA受体的调节以及Stargazin磷酸化的功能的相互依存关系，这项工作对学习和记忆的机制具有广泛的影响。