Changes in anesthetic requirements after spinalization

脊柱化术后麻醉要求的变化

• 批准号: 6626204
• 负责人: STEVEN L JINKS
• 金额: $ 4.64万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6626204
• 关键词: anesthesia; anesthetics; autonomic reflex; blood pressure; dizocilpine; dorsal horn; dosage; electrophysiology; excitatory aminoacid; inhibitor /antagonist; isoflurane; laboratory rat; limb movement; pain; postdoctoral investigator; psychomotor function; spinal cord injury; spinal reflex; stimulus /response; sympathetic nervous system

Reorganization of spinal circuitry and removal of supraspinal influences after spinal cord injury (SCI) result in pathophysiologic conditions, such as motor and autonomic hyperflexia (AH) in response to noxious stimuli. Individuals with SCI are thus at increased risk during surgical procedures and require special anesthetic management, but few studies that have addressed this problem. An animal model to assess changes in anesthetic effects on spinal reflexes following SCI would aid in developing effective anesthetic management of the SCI population. Changes in anesthetic (isoflurane) effects on motor and sympathetic reflex pathways after acute and chronic spinalization, and the effects of NMDA antagonists on these changes, will be investigated in rats. These will be studied using quantitative measurement of responses evoked by noxious stimuli (pinch, heat), including limb movement, blood pressure, and electrophysiological recording of single dorsal horn neurons, along with simultaneous recording of sympathetic and motor nerve activity. Changes in isoflurane dose-response functions for these parameters., and correlations among them, will be determined before and after acute (complete and partial) and chronic spinalization. Thus, these studies aim to determine how and where changes in spinal reflex pathways occur after SCI, and how they relate to anesthetic requirements. This knowledge will lead to safer anesthesia in SCI patients, and to a better understanding of nociceptive/spinal reflex pathways occur after SCI, and how they relate to anesthetic requirements. This knowledge will lead to safer anesthesia in SCI patients, and to a better understanding of nociceptive/spinal reflex pathways, their supraspinal control, and changes in spinal circuitry following SCI.

脊髓损伤(SCI)后脊髓回路的重组和棘上影响的消除导致了诸如对伤害性刺激反应的运动和自主神经功能亢进(AH)等病理生理状况。因此，患有脊髓损伤的患者在手术过程中风险增加，需要特殊的麻醉处理，但很少有研究解决这一问题。建立一个动物模型来评估脊髓损伤后麻醉对脊髓反射的影响，将有助于发展脊髓损伤人群的有效麻醉管理。在大鼠急性和慢性脊髓化后，将观察麻醉剂(异氟醚)对运动和交感反射通路影响的变化，以及NMDA拮抗剂对这些变化的影响。这些将通过定量测量伤害性刺激(挤压、热)引起的反应，包括肢体运动、血压和单个背角神经元的电生理记录，以及同时记录交感神经和运动神经活动来研究。这些参数的异氟醚剂量反应函数的变化以及它们之间的相关性，将在急性(完全和部分)和慢性脊髓化之前和之后确定。因此，这些研究的目的是确定脊髓损伤后脊髓反射通路的改变是如何发生的以及在哪里发生，以及它们与麻醉需求的关系。这些知识将使脊髓损伤患者的麻醉更安全，并更好地了解脊髓损伤后发生的伤害性/脊髓反射通路，以及它们与麻醉要求的关系。这些知识将使脊髓损伤患者获得更安全的麻醉，并更好地了解伤害性/脊髓反射通路、脊髓上控制以及脊髓损伤后脊髓回路的变化。