ENHANCEMENT OF MOLECULAR BIOLOGY RESEARCH FACILITIES

描述(由申请人提供)： 2002年2月，伊利诺伊大学获得了美国国立卫生研究院(NIH)的资金，以建立COBRE。这笔资金与计算生物学、计算机科学和生物学方面的专业知识相结合，以调查各种突变过程在适应性进化的节奏和轨迹上的相对重要性，以及在进化过程中出现的模式的功能意义，并开发出提高理解蛋白质、结构基因和整个基因组中功能域进化历史的能力的计算方法。来自NIH Cobre计划的资金已被用于在UI建立进化过程研究中心(CREPE)。所要求的补充资金是为了加强作为CREPE的组成部分而提出的实验进化实验的完成和扩展。具体来说，这笔资金将用于：1)翻新教学实验室空间，以研究将用于扩建核心分子生物学设施的实验室空间；2)扩大核心分子生物学设施的能力，用于DNA文库建设和筛选、DNA测序以及Southern和Northern杂交分析；以及3)获得在原核生物种群实验进化研究中有用的设备。

DESCRIPTION (provided by applicant): In February 2002, the UI received funding from the National Institutes of Health (NIH) to establish a COBRE. The funding brings together with expertise in computational biology, computer science and biology to investigate the relative importance of various mutagenic processes on the tempo and trajectory of adaptive evolution, the functional significance of patterns that emerge during the course of evolution, and to develop computational approaches that improve ability to understand the evolutionary history of functional domains in proteins, structural genes, and entire genomes. The funding from the NIH COBRE program has been used to establish the Center for Research on Processes in Evolution (CRePE) at the UI. Requested supplemental funding are to enhance completion and extension of the experimental evolution experiments proposed as an integral part of CRePE. Specifically the funds will be used to: 1) renovate teaching laboratory space to research laboratory space that will be used for expansion of the Core Molecular Biology facility, 2) expand the capabilities of the Core Molecular Biology facility for DNA library construction and screening, DNA sequencing, as well as Southern and Northern hybridization analyses, and 3) acquire equipment useful in studies on the experimental evolution of prokaryotic populations.