COBRE: UID: DNA SEQUENCE ANALYSIS CORE FACILITY

COBRE:UID:DNA 序列分析核心设施

基本信息

  • 批准号:
    8167450
  • 负责人:
  • 金额:
    $ 17.14万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-02-01 至 2011-01-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The tools used for high throughput DNA sequencing, comparative genomics, SNP analysis, and the analysis of gene expression have become nearly indispensable in contemporary biomedical research. As a result of our current COBRE support we have been able to establish a high throughput multi-user DNA Sequence Analysis Core facility; the only one at the University of Idaho. This facility has greatly benefited both COBRE researchers and the larger biomedical research community at the University. In order for the University to remain competitive in biomedical research, and for COBRE funded researchers to achieve the aims of the proposed research projects, we must not only maintain the current capabilities of the core, but also add the capability to rapidly do whole genome re-sequencing and gene expression analysis. The existing DNA Sequence Analysis Core Facility provides researchers access to the instruments, equipment, and facilities needed for the analysis of nucleic acid sequences. The Core Facility occupies 850 sq. feet of newly renovated space in Life Sciences South. It is equipped with the major instrumentation and computational resources described below, as well as wet lab benches, biosafety cabinets, thermocyclers, centrifuges, microfuges, freezers, refrigerators, and other minor equipment needed for sample preparation. A portion of the laboratory is dedicated to data analysis and bioinformatics and has high-end workstations and printers that are linked via the internet to the software and hardware resources of the Bioinformatics Core Facility on campus. A technician oversees operation and maintenance of the instruments, and provides instruction, guidance, and trouble-shooting assistance to users of the facility. The major instrumentation now available in the Core Facility includes the following: Nimblegen Hybridization System. The Hybridization system is a four-bay system that can accommodate multiple array types to achieve consistent and reproducible microarray hybridization results GenePix 4000B Microarray Scanner. The GenePix Scanner is capable of scanning and quantifying DNA microarray slides from multiple different vendors including Nimblegen and Agilant microarrays Genome Sequencer FLX Titanium Series Instrument. The Genome Sequencer FLX Instrument, powered by GS FLX Titanium and Standard series reagents, features a groundbreaking combination of long reads, exceptional accuracy and high throughput. Applications include de novo sequencing, resequencing of whole genomes and target DNA regions, metagenomics and RNA analysis. + Applied Biosystems PRISM 3730 DNA Analyzer. The ABI 3730 is a 48 capillary system that can accommodate two array lengths, 50cm and 36cm for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing + Applied Biosystems PRISM 3100 DNA Analyzer. The ABI 3100 is a fluorescence based DNA analysis system in which 16 electrophoresis capillaries are operated in parallel. The instrument is almost exclusively used for DNA fragment analysis. + Qiagen BioRobot 3000. The Qiagen BioRobot allows for high throughput sample processing using a 96-well microplate format. The customized workstation is equipped with a 4 microplate shaker system for efficient cell resuspension or lysis, two vacuum manifolds for simultaneous purification of 192 samples, a high-speed pipetting system with four dilutor units, one cooling and heating Peltier system for performing heat-dependent steps, and a T-grip robotic arm for moving items such as microplates between locations on the worktable. + Two Macintosh Power Mac G5 computers both of which have dual 2GHz processors with 512kb of cache per processor. For memory and storage each computer is equipped with 1GB of RAM, two 250GB hard drives and a Pioneer DVD-RW/CD-RW disc drive. This computer is connected to the high speed IBEST network with an internal gigabit Ethernet network card. + One Macintosh Mac Pro computer with two quad-Core Intel Xeon 2.8GHz processors with 12MB of cache per processor. For memory and storage it is equipped with 4GB of RAM, two 500GB hard drives and a Pioneer DVD-RW/DVR disc drive. This computer is connected to the high speed IBEST network with an internal gigabit Ethernet network card. + One Dell Computer with a Intel Pentium 4 CPU 1.6GHz processor with 256kb of cache For memory and storage it is equipped with 256MB of RAM, a 76GB hard drive and a CD-R/CD-RW disc drive. It is setup to run Ubuntu Linux and is also connected to the high speed IBEST network. + One Dell Computer with an Intel Pentium 4 3GHz processor. For memory and storage it is equipped with 1GB of RAM, a 300GB hard Drive and a DVD/CD-R disc drive. It is currently running Windows XP and is also connected to the high speed IBEST network. One Dell Computer with Dual Intel Xeon 2.26 Ghz Quad Core Processors. For memory and storage it is equiped with 24GB of RAM and 1TB Hard Drive and DVD/CD-R disc drive. It is currently running Ubuntu Linux 9.04 and is also connected to the high speed IBEST network.
这个子项目是众多研究子项目之一

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

LARRY J FORNEY其他文献

LARRY J FORNEY的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('LARRY J FORNEY', 18)}}的其他基金

Host-Microbiota Interactions and Chlamydia Trachomatis Infection Outcomes
宿主-微生物群相互作用和沙眼衣原体感染结果
  • 批准号:
    10239625
  • 财政年份:
    2021
  • 资助金额:
    $ 17.14万
  • 项目类别:
Host-Microbiota Interactions and Chlamydia Trachomatis Infection Outcomes
宿主-微生物群相互作用和沙眼衣原体感染结果
  • 批准号:
    10449336
  • 财政年份:
    2021
  • 资助金额:
    $ 17.14万
  • 项目类别:
Elucidating causes of vaginal symptoms using a multi-omics approach
使用多组学方法阐明阴道症状的原因
  • 批准号:
    9883844
  • 财政年份:
    2016
  • 资助金额:
    $ 17.14万
  • 项目类别:
Elucidating causes of vaginal symptoms using a multi-omics approach
使用多组学方法阐明阴道症状的原因
  • 批准号:
    9273625
  • 财政年份:
    2016
  • 资助金额:
    $ 17.14万
  • 项目类别:
Elucidating causes of vaginal symptoms using a multi-omics approach - Admin. Supplement
使用多组学方法阐明阴道症状的原因 - 管理员。
  • 批准号:
    10091585
  • 财政年份:
    2016
  • 资助金额:
    $ 17.14万
  • 项目类别:
Development of the vaginal microbiome in young Black women
年轻黑人女性阴道微生物组的发育
  • 批准号:
    8898465
  • 财政年份:
    2014
  • 资助金额:
    $ 17.14万
  • 项目类别:
COBRE Phase III: Transitional Center for Research on Processes in Evolution
COBRE 第三阶段:进化过程研究过渡中心
  • 批准号:
    8796191
  • 财政年份:
    2013
  • 资助金额:
    $ 17.14万
  • 项目类别:
COBRE Phase III: Transitional Center for Research on Processes in Evolution
COBRE 第三阶段:进化过程研究过渡中心
  • 批准号:
    8304605
  • 财政年份:
    2013
  • 资助金额:
    $ 17.14万
  • 项目类别:
COBRE Phase III: Transitional Center for Research on Processes in Evolution
COBRE 第三阶段:进化过程研究过渡中心
  • 批准号:
    9213373
  • 财政年份:
    2013
  • 资助金额:
    $ 17.14万
  • 项目类别:
COBRE: UID: PROGRAM ADMINISTRATION & FACULTY MENTORING
COBRE:UID:项目管理
  • 批准号:
    8359575
  • 财政年份:
    2011
  • 资助金额:
    $ 17.14万
  • 项目类别:

相似海外基金

Conference: Global Bioinformatics Education Summit 2024 — Energizing Communities to Power the Bioeconomy Workforce
会议:2024 年全球生物信息学教育峰会 — 激励社区为生物经济劳动力提供动力
  • 批准号:
    2421267
  • 财政年份:
    2024
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Standard Grant
Conference: The 9th Workshop on Biostatistics and Bioinformatics
会议:第九届生物统计与生物信息学研讨会
  • 批准号:
    2409876
  • 财政年份:
    2024
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Standard Grant
Open Access Block Award 2024 - EMBL - European Bioinformatics Institute
2024 年开放获取区块奖 - EMBL - 欧洲生物信息学研究所
  • 批准号:
    EP/Z532678/1
  • 财政年份:
    2024
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Research Grant
PAML 5: A friendly and powerful bioinformatics resource for phylogenomics
PAML 5:用于系统基因组学的友好且强大的生物信息学资源
  • 批准号:
    BB/X018571/1
  • 财政年份:
    2024
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Research Grant
PDB Management by The Research Collaboratory for Structural Bioinformatics
结构生物信息学研究合作实验室的 PDB 管理
  • 批准号:
    2321666
  • 财政年份:
    2024
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Cooperative Agreement
Building a Bioinformatics Ecosystem for Agri-Ecologists
为农业生态学家构建生物信息学生态系统
  • 批准号:
    BB/X018768/1
  • 财政年份:
    2023
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Research Grant
Integrative viral genomics and bioinformatics platform
综合病毒基因组学和生物信息学平台
  • 批准号:
    MC_UU_00034/5
  • 财政年份:
    2023
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Intramural
Collaborative Research: IIBR: Innovation: Bioinformatics: Linking Chemical and Biological Space: Deep Learning and Experimentation for Property-Controlled Molecule Generation
合作研究:IIBR:创新:生物信息学:连接化学和生物空间:属性控制分子生成的深度学习和实验
  • 批准号:
    2318829
  • 财政年份:
    2023
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Continuing Grant
Planning Proposal: CREST Center in Bioinformatics
规划方案:CREST生物信息学中心
  • 批准号:
    2334642
  • 财政年份:
    2023
  • 资助金额:
    $ 17.14万
  • 项目类别:
    Standard Grant
Bioinformatics Core
生物信息学核心
  • 批准号:
    10404414
  • 财政年份:
    2023
  • 资助金额:
    $ 17.14万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了