Integrative Tissue Engineering in the Spinal Cord

脊髓整合组织工程

基本信息

  • 批准号:
    6689225
  • 负责人:
  • 金额:
    $ 3.82万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-07-21 至 2006-07-20
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): An integrative, tissue-engineering approach to treat spinal cord injury will be evaluated in a rat transection model. A biodegradable implant with controlled microarchitecture will be developed and fabricated from poly(lactic-co-glycolic acid) (PLGA). Implant architecture will be evaluated and characterized with scanning electron microscopy, confocal microscopy, and X-ray micro-computed tomography. Chondroitinase ABC (C-ABC), an enzyme shown to break down the proteoglycan-rich scar that results from injury, will be encapsulated in PLGA microspheres, and its bioactivity and release kinetics will be evaluated in vitro. Techniques for incorporating C-ABC into the polymer implant will be investigated, and the distribution and release of C-ABC from the implant will be described. Schwann cells will be harvested from neonatal rat pup sciatic nerves, expanded in cell culture, and suspended in Matrigel for incorporation into the polymer implant. Adult Sprague-Dawley rats will be anesthetized before receiving thoracic 8-9 laminectomies and spinal cord transections. Various implants will be inserted into the spinal canals and the dura closed over them. Spinal cords will be harvested at multiple time points, split longitudinally, and fixed for histology, immunohistochemistry, and scanning electron microscopy (SEM). Morphologic evaluation of tissue repair will be performed on tissue sections, while functional assays will be employed for assessment of neurological recovery. Experimental groups and controls will be evaluated for determining the effects of each implant component, as well as their various combinations, on axon regeneration and functional recovery.
描述(由申请人提供):将在大鼠横断模型中评价治疗脊髓损伤的综合性组织工程方法。一种具有可控微结构的生物可降解植入物将由聚乳酸-羟基乙酸共聚物(PLGA)开发和制造。将使用扫描电子显微镜、共聚焦显微镜和X射线显微计算机断层扫描对植入物结构进行评价和表征。软骨素酶ABC(C-ABC),一种被证明能分解由损伤引起的富含蛋白聚糖的瘢痕的酶,将被封装在PLGA微球中,并将在体外评价其生物活性和释放动力学。将C-ABC纳入聚合物植入物的技术进行了研究,并将描述C-ABC从植入物的分布和释放。将从新生大鼠幼仔坐骨神经中收获许旺细胞,在细胞培养物中扩增,并悬浮在基质胶中以掺入聚合物植入物中。成年Sprague-Dawley大鼠将在接受胸部8-9椎板切除术和脊髓横断术之前麻醉。各种植入物将被插入椎管内,并在其上封闭硬脑膜。将在多个时间点采集脊髓,纵向切开并固定,用于组织学、免疫组织化学和扫描电子显微镜(SEM)检查。将在组织切片上进行组织修复的形态学评价,而功能测定将用于评估神经恢复。将对实验组和对照组进行评价,以确定每种植入物组分及其各种组合对轴突再生和功能恢复的影响。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Michael J Moore其他文献

Novel high-throughput human cells-based co-culture screen for chemotherapeutics induced neurotoxicity.
针对化疗药物引起的神经毒性的新型高通量人类细胞共培养筛选。
Diagnostic accuracy and clinical utility of a simplified low cost method of counting CD4 cells with flow cytometry in Malawi: diagnostic accuracy study
马拉维流式细胞术计数 CD4 细胞的简化低成本方法的诊断准确性和临床实用性:诊断准确性研究
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    C. MacLennan;G. van;Oosterhout;A. Sarah;White;F. Simukonda;J. Bwanali;Michael J Moore;E. Zijlstra;M. Drayson;M. Molyneux
  • 通讯作者:
    M. Molyneux
Nerve-on-a-chip platform for assessing chemotherapy-induced peripheral neuropathy.
用于评估化疗引起的周围神经病变的神经芯片平台。
Morphology of nares associated with stereo-olfaction in baleen whales
与须鲸立体嗅觉相关的鼻孔形态
  • DOI:
    10.1098/rsbl.2023.0479
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    3.3
  • 作者:
    Conor Ryan;M. C. I. Martins;Kevin Healy;Lars Bejder;Salvatore Cerchio;Fredrik Christiansen;John Durban;H. Fearnbach;Sarah M E Fortune;Ari S. Friedlaender;William R Koski;Carolyn Miller;Fabian M Rodríguez;P. Segre;J. Urbán R.;F. Vivier;Caroline R. Weir;Michael J Moore
  • 通讯作者:
    Michael J Moore

Michael J Moore的其他文献

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{{ truncateString('Michael J Moore', 18)}}的其他基金

Development and Maintenance of Human and Animal Food Rapid Response Teams (U2F) Massachusetts FPP
人类和动物食品快速反应小组 (U2F) 的开发和维护 马萨诸塞州 FPP
  • 批准号:
    10828091
  • 财政年份:
    2023
  • 资助金额:
    $ 3.82万
  • 项目类别:
Flexible Funding Model-Infrastructure Maintenance for State Manufactured Food Regulatory Programs in Massachusetts
灵活的资助模式——马萨诸塞州国家制造食品监管项目的基础设施维护
  • 批准号:
    10828088
  • 财政年份:
    2023
  • 资助金额:
    $ 3.82万
  • 项目类别:
Food Protection Program Maintenance and Integration of Rapid Response and Manufactured Food Regulatory Program Standards
食品保护计划快速响应和制成品监管计划标准的维护和整合
  • 批准号:
    10213038
  • 财政年份:
    2018
  • 资助金额:
    $ 3.82万
  • 项目类别:
Food Protection Program Maintenance and Integration of Rapid Response and Manufactured Food Regulatory Program Standards
食品保护计划快速响应和制成品监管计划标准的维护和整合
  • 批准号:
    10458486
  • 财政年份:
    2018
  • 资助金额:
    $ 3.82万
  • 项目类别:
Localized immobilization of ephrin-B2 for neurite guidance in 3D culture
肝配蛋白-B2 的局部固定用于 3D 培养中的神经突引导
  • 批准号:
    7849020
  • 财政年份:
    2009
  • 资助金额:
    $ 3.82万
  • 项目类别:
Integrative Tissue Engineering in the Spinal Cord
脊髓整合组织工程
  • 批准号:
    6789368
  • 财政年份:
    2003
  • 资助金额:
    $ 3.82万
  • 项目类别:

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