Interplay of Microglia & Alloreactive CTL-Damaged Glioma

小胶质细胞的相互作用

• 批准号: 6585033
• 负责人: NISHA VIRASCH
• 金额: $ 2.56万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6585033
• 关键词: clinical research; cytotoxic T lymphocyte; dexamethasone; glioma; human tissue; leukocyte activation /transformation; macrophage; microglia; mixed tissue /cell culture; neoplasm /cancer immunology; phagocytes; phosphatidylserines; predoctoral investigator

DESCRIPTION (provided by applicant): The prognosis for primary malignant brain tumors remains poor. In a Phase I trial, one specific type of effector cell, alloreactive cytotoxic T lymphocytes (CTL), have shown promise. Study of glioma cells, damaged by treatment with alloreactive CTL, with other auxillary brain cells such as microglia/macrophages in a reconstituted system may help us to better understand other complex interactions involved in situ. We hypothesize that after co-incubating glioma cells with alloreactive CTL, the resulting damaged (apoptotic/necrotic) gliomas will induce microglia/macrophage phagocytic function. We will also determine the effects of dexamethasone on the phagocytic function of microglia/macrophages. Lastly, we hypothesize that after co-incubating glioma cells with alloreactive CTL, the resulting damaged gliomas will induce microglia/macrophage activation.

描述（由申请人提供）：原发性恶性脑肿瘤的预后仍然很差。在一项 I 期试验中，一种特定类型的效应细胞，即同种异体反应性细胞毒性 T 淋巴细胞 (CTL)，已显示出前景。对因同种异体反应性 CTL 治疗而受损的神经胶质瘤细胞与重建系统中的其他辅助脑细胞（如小胶质细胞/巨噬细胞）的研究可能有助于我们更好地理解原位涉及的其他复杂相互作用。我们假设神经胶质瘤细胞与同种异体反应性 CTL 共孵育后，所产生的受损（凋亡/坏死）神经胶质瘤将诱导小胶质细胞/巨噬细胞吞噬功能。我们还将确定地塞米松对小胶质细胞/巨噬细胞吞噬功能的影响。最后，我们假设神经胶质瘤细胞与同种异体反应性 CTL 共孵育后，产生的受损神经胶质瘤将诱导小胶质细胞/巨噬细胞激活。