Cloning of a Hookworm Excretory-Secretory Protein

钩虫排泄蛋白的克隆

• 批准号: 6622327
• 负责人: RICHARD D BUNGIRO
• 金额: $ 4.81万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-10 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6622327
• 关键词: Nematoda; active immunization; anemia; animal extract; antibody; complementary DNA; disease /disorder model; gene expression; hamsters; host organism interaction; immunity; immunocytochemistry; laboratory rabbit; molecular cloning; northern blottings; parasitic gastrointestinal disorder; parasitism; passive immunization; pathologic process; polymerase chain reaction; protein biosynthesis; protein purification; protein structure function; recombinant proteins; secretory protein; western blottings

DESCRIPTION: provided by the candidate): Hookworms are a major global public health problem, infecting over a billion people worldwide. Blood feeding adult hookworms are a leading cause of anemia and malnutrition in developing countries, extracting a particularly devastating toll on children and women of childbearing age. Adult worms secrete numerous factors at the site of attachment in the intestine that are likely to contribute to disease pathogenesis. Accordingly, the initial aims of this project are the molecular cloning, expression, and characterization of AcES-1, a novel excretory-secretory protein which ahs been isolated from the human hookworm Ancylostoma ceylanicum. Using an animal model of hookworm infection the role of AcES-1 in pathogenesis will then be examined using active and passive immunization techniques. Aside from shedding light in the nature of the host-parasite interaction, study of factors such as AcES-1 may ultimately yield novel targets for immunological or pharmacological intervention to reduce the burden of hookworm disease.

描述：由候选人提供）：钩虫是一种主要的全球公共 健康问题，感染了全球超过十亿人。吸血成虫 钩虫是发展中国家贫血和营养不良的主要原因 这些国家的儿童和妇女遭受了特别惨重的损失， 生育年龄蠕虫在分泌部位分泌多种因子， 肠内附着物可能导致疾病 发病机制因此，该项目的最初目标是分子 新型AcES-1的克隆、表达和表征 从人钩虫中分离出的一种排泄分泌蛋白 锡兰钩虫使用钩虫感染的动物模型， AcES-1的发病机制，然后将检查使用主动和被动 免疫技术。除了揭示了 宿主-寄生虫相互作用，对AcES-1等因子的研究可能最终产生 免疫学或药理学干预的新靶点， 钩虫病的负担。