Role of PKA Cbeta Subunits in Learning and Memory

描述：（改编自申请人摘要）：拟议的研究涉及缺乏蛋白激酶A （PKA）所有C13亚基的小鼠的产生。这些小鼠将被用于研究PKA亚基在学习和记忆中的作用。PKA是学习和记忆过程中必不可少的酶，而Cb亚基在已知对建立长期记忆至关重要的大脑区域中表达。将在空间和恐惧学习任务中测试缺乏Cb的小鼠，以确定Cb在这些类型的记忆中的重要性。学习和记忆的分子机制将通过确定敲除Cb对被认为对学习和记忆重要的信号转导和基因表达事件的影响来研究。最后，利用转基因方法试图挽救Cb敲除小鼠的表型。如果我们要减少、逆转甚至预防与老年和神经系统疾病相关的记忆丧失，详细了解学习和记忆的分子机制是必要的。

DESCRIPTION: (Adapted from applicant's abstract): The proposed research involves the generation of mice lacking all C13 subunits of protein kinase A (PKA). These mice will be utilized in studies examining the role of this PKA subunit in learning and memory. PKA is an essential enzyme in the process of learning and memory, and the Cb subunit is expressed in regions of the brain known to be critical for the establishment of long-term memory. Mice lacking Cb will be tested in spatial and fear learning tasks to determine the significance of Cb in these types of memory. The molecular mechanism of learning and memory will be examined by determining the effect of knocking out Cb on signal transduction and gene expression events thought to be important for learning and memory. Finally, a transgenic approach will be utilized to attempt to rescue the phenotype of the Cb knockout mice. A detailed understanding of the molecular mechanism of learning and memory is necessary if we are to reduce, reverse or even prevent the memory loss associated with old age and neurological disease.